摘要
建立了高效液相色谱（HPLC）快速测定土曲霉发酵液中衣康酸及残糖的分析方法.使用TOSOH TskgeloApak-A色谱柱,以0.75 mmol/L H2SO4为流动相,在柱温45℃,流速1.0 mL/min的条件下,采用示差折光检测器进行检测.结果表明,该方法在20 min内可实现发酵液中衣康酸和葡萄糖的完全分离与定量.衣康酸在0 ～ 10.056 g/L范围内的线性关系良好,相关系数为0.999 9,相对标准偏差（n=6）为0.33％,加标回收率100.88％ ～ 102.74％.葡萄糖在0～6.016 g/L范围内的线性关系良好,相关系数为0.9999,相对标准偏差（n=6）为0.12％,加标回收率101.26％～ 103.12％.该法适用于土黄霉发酵液中衣康酸和葡萄糖的快速、高效分离和定量测定.
A high performance liquid chromatographic method for rapid determination of itaconic acid and saccharides in itaeonie acid fermentation broth of Aspergillus terreus was developed. A TOSOH Tskgel oApak-A column was used at 45 ~C and the mobile phase was 0.75 mmol/L H2SO4 at a flow rate of 1.0 mL/min. Under the optimized conditions. The itaconic acid and glucose in samples were determined and completely separated within 20 min by a refractive index detector （RID）. The Linear correlation coefficient for itaconic acid was 0.999 9 in the range of 0 - 10.056 g/L. The Linear cor- relation coefficient for glucose was 0. 999 9 in the range of 0 - 6. 016 g/L. The relative standard deviation （ RSD,n =6） of itaconic acid and glucose was 0, 33% and 0.15% respectively. The recovery rate was 100.88% - 102.74% and of 101.26% - 103.12% separately. This method established is proved to be fast and accurate for the quantitative analysis of the itaconic acid and glucose in fermen- tation broth of Aspergillus terreus.
出处
《江西科学》
2014年第6期865-868,共4页
Jiangxi Science
关键词
高效液相色谱
衣康酸
葡萄糖
土曲霉
发酵液
high performance liquid chromatography（ HPLC ）
itaconic acid
glucose
Aspergillus ter-reus
fermentation broth
