摘要
以桂黔吊石苣苔的茎段和叶片为外植体材料,对桂黔吊石苣苔进行离体培养与快速繁殖技术研究。结果表明:桂黔吊石苣苔茎段腋芽可直接诱导萌发,在MS+6-BA 1.0 mg·L-1+IBA 0.1 mg·L-1培养基上萌发率最高,达75%;适宜的继代增殖及壮苗培养基为1/2MS+NAA0.2 mg·L-1,繁殖系数为6.0/60 d,继代培养中茎段外植体可以诱导出愈伤组织,但诱导率较低,未能进一步分化成苗;最佳生根培养基为1/2MS+6-BA 0.2mg·L-1+NAA 0.8 mg·L-1+AC 0.1%+香蕉5%,生根率达100%;在大棚中模拟桂黔吊石苣苔自然生境对生根苗进行移栽,成活率在95%以上。
Tissue culture and rapid propagation technique of Lysionotus aeschynanthoides were studied.The results showed that the axillary buds of Lysionotus aeschynanthoides could directly germinate in MS＋6-BA 1.0 mg·L-1 ＋IBA 0.1 mg·L-1 and the germination rate was 75%.The primary culture medium for bud induction from stem seg-ments was MS＋6-BA 1.0 mg·L-1 ＋IBA 0.1 mg·L-1 .The subculture medium for bud multiplication and seedlings growing was 1/2 MS＋ NAA 0.2 mg · L-1 ,and proliferation coefficient was 6.0/60 d.Callus could be inducted through the pedicels explants in subculture but could not develop further.The optimal rooting medium was 1/2 MS＋BA 0.2 mg·L-1 ＋NAA 0.8 mg·L-1 ＋AC 0.1% ＋5% banana juice,and rooting rate was 100%.The rooting pla-ntlets were transplanted in greenhouse that simulated the natural growing environment of L .aeschynanthoids ,the survival rate was more than 95%.
出处
《广西植物》
CAS
CSCD
北大核心
2014年第6期874-878,共5页
Guihaia
基金
广西科技攻关项目(0992003B-31)
国家自然科学基金(31160055)
关键词
桂黔吊石苣苔
组织培养
快速繁殖
Lysionotus aeschynanthoides
tissue culture
rapid propagation
