摘要
目的探讨细菌脂多糖免疫对炎症性肠病小鼠肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)表达的影响及其作用机制。方法 BALB/C雌性小鼠10只随机分为实验组5只和对照组5只,实验组腹腔内注射1.0mg/kg细菌脂多糖和免疫佐剂的混合物,对照组腹腔内注射免疫佐剂1.0mg/kg,2组小鼠均每周注射1次,注射4次。末次注射1周后均给予150mg/kg的三硝基苯磺酸乙醇溶液灌肠诱导炎症性肠病。观察2组小鼠一般状态、体质量改变情况、粪便性状,检测粪便隐血;灌肠1周后处死小鼠,观察结肠组织学改变,采用ELISA法检测结肠组织TNF-α表达情况,采用Western blot法检测结肠组织核因子(nuclear factor,NF)-κB表达情况。结果 2组小鼠灌肠后第2天均出现懒动、厌食、皮毛凌乱、腹泻,第3天出现血便、体质量下降,实验组上述表现较对照组轻,恢复快;实验组小鼠第3天体质量下降克数((1.60±0.38)g)、粪便隐血阳性持续时间((2.40±0.54)d)、结肠组织学评分((3.72±2.45)分)、结肠组织TNF-α表达水平((20.16±3.21)ng/L)和NF-κB吸光度值((18.43±5.36)×10-3)均明显低于对照组((2.80±0.45)g、(3.60±0.46)d、(6.39±1.72)分、(32.35±2.80)ng/L、(25.80±3.79)×10-3)(P<0.05)。结论细菌脂多糖免疫可降低炎症性肠病小鼠结肠组织TNF-α和NF-κB表达水平,减轻结肠组织病理损伤,机体对细菌脂多糖反应异常可能是炎症性肠病的发病因素之一。
Objective To investigate the effects of bacterial lipopolysaccharide immune on the expression of tumor necrosis factor-α（TNF-α）and its mechanism in mice with inflammatory bowel disease.Methods Ten BALB/C mice were randomly divided into experimental group and control group,with 5 mice in each group.Experiment group received intraperitoneal injection of 1.0 mg/kg bacterial lipopolysaccharide immunologic adjuvant mixtures once a week.Control group only received intraperitoneal injection of 1.0 mg/kg immunologic adjuvant.Both groups were given injection for four times.The inflammatory bowel disease was induced by clysis with 150 mg/kg trinitro-benzene-sulfonic acid in ethanol solution one week after the last injection.The general status,the change of body mass,and fecal features of mice were observed in both groups.The fecal occult blood was tested.The mice were sacrificed one week after clysis.The histological changes in the colon were evaluated by HE staining.The expression of TNF-αin the colonic tissue was detected by ELISA,and the expression of NF-κB was detected by Western blot.Results Both two groups showed accidie,inappetence,messy fur and diarrhea on the second day after clysis,followed by bloody stools and body mass loss on the third day,which were milder and recovered more quickly in experiment group in comparison with control group.On the third day,the body mass loss,the duration of positive fecal occult blood,the histopathologic score of colon,the level of TNF-α,and the level of NF-κB were（1.60±0.38）g,（2.40±0.54）days,3.72±2.45,（20.16±3.21）ng/L and（18.43±5.36）×10^-3 in experiment group,significantly lower than those in control group（（2.80±0.45）g,（3.60±0.46）days,6.39±1.72,（32.35±2.80）ng/L,（25.80±3.79）×10^-3）（P〈0.05）.Conclusion The bacterial lipopolysaccharide immune can decrease the expressions of TNF-αand NF-κB and reduce the pathological damage to the colonic tissue in mice with inflammatory bowel disease. The abnormal reaction of the body against bacterial lipopolysaccharide may be one of the pathogenic factors of inflammatory bowel disease.
出处
《中华实用诊断与治疗杂志》
2015年第1期28-30,共3页
Journal of Chinese Practical Diagnosis and Therapy
基金
大连市卫生局基金(2013192)
