摘要
目的 观察土三七在建立的肝小静脉闭塞（HVOD）大鼠模型中对血液系统毒性和血管内皮分泌功能的影响,为临床治疗土山七所致的HVOD提供依据和思路.方法 取60只SD大鼠,雌雄各半,分成5组,设空白对照组（蒸馏水20 ml/kg）,土三七低剂量组（5 g/kg）、中剂量组（10 g/kg）、高剂量组（20 g/kg）和三七对照组（10 g/kg）,连续给药4周.观察土三七对大鼠血细胞分类、凝血功能、血管内皮分泌功能及脾脏组织病理的影响.组间比较采用方差分析（One-WayANOVA）.结果 给药4周后,土三七各组大鼠体质量降低;白细胞数、中性粒细胞数、淋巴细胞数、单核细胞数和嗜酸粒细胞数均显著升高;血小板数和血小板压积显著降低,平均血小板体积和血小板分布宽度均显著升高;血液凝血酶原时间、活化部分凝血酶原时间、凝血酶时间、凝血酶原比值、凝血酶原国际标准化比值和纤维蛋白原时间均明显升高,血小板压积、纤维蛋白原和血小板聚集率显著降低;内皮素和一氧化氮水平均显著升高;与空白对照组比较,均P＜0.01或P＜0.05.脾脏中脾小结明显减少,未见生发中心,大量淋巴细胞弥漫分布,红髓血窦减少. 结论 土三七对正常大鼠血液系统有一定毒性作用,能降低血小板和血小板压积,抑制血液凝血时间和血小板聚集作用,提高血管内皮分泌功能,减少脾脏中脾小结和生发中心.目前临床治疗土山七所致HVOD的观点,中药以活血化瘀、西药以抗凝抗血小板聚集为主的治疗方案是否妥当值得商榷.
Objective To observe the effects ofGynura segetum in rats with hepatic veno-occlusive disease （HVOD）.Methods Sixty Sprague-Dawley rats were assigned to a blank control group,one of three Gynura segetum treatment groups （low-dose group,5.0 g/kg; mid-dose group,10 g/kg; high-dose group,20 g/kg）,or a pseudo-drug group （10 g/kg of pseudo-ginseng）.After 28 days of treatment,effects on white blood cell count,coagulation,secreted factors from vascular endothelium,and histopathology of the spleen were observed and inter-group differences were statistically assessed.Results After the 4-week administration,all rats in the Gynura segetum treatment groups showed decrease in body weight,increases in numbers of leukocytes,neutrophils,lymphocytes,monocytes and eosinophils.decreases in platelets and platelet hematocrit,and increases in mean platelet volume and platelet distribution.In addition,the Gynura segetum treatments increased the prothrombin time,activated partial thromboplastin time,thrombin time,prothrombin ratio and international normalized ratio,but decreased the PT％,fibrinogen level and platelet aggregation.Serum levels of endothelin and nitric oxide were also elevated by the Gynura segetum treatments.All measured parameters showed significant differences from the control group （P ＜ 0.01 or ＜ 0.05）.Finally,the splenic follicles were significantly reduced and the spleens showed an absence of germinal centers along with a large number of diffuse lymphocytes and reduced red pulp sinusoids.Conclusion The Gynura segetum treatment has some toxic effects; it can reduce platelet count and platelet hematocrit,inhibit blood clotting time and platelet aggregation,increase the secretion of factors from the vascular endothelium and disrupt spleen histology.
出处
《中华肝脏病杂志》
CAS
CSCD
北大核心
2015年第1期59-63,共5页
Chinese Journal of Hepatology
关键词
土三七
菊科
肝小静脉闭塞性疾病
血液系统毒性
内皮分泌功能
Gynura segetum
Composite family
Hepatic venular occlusive disease
Hematologic toxicity
Secretion of vascular endothelial
