摘要
目的探讨无患子及其制剂的鉴别方法。方法采用薄层色谱法在不同条件下进行薄层色谱考察,选择适宜的薄层色谱条件。结果最佳薄层色谱条件为:以正丁醇-乙酸乙酯-水-浓氨水(40∶10∶50∶1;V∶V∶V∶V)上层液为展开剂,室内环境中展开,晾干,喷5%磷钼酸乙醇液,105℃加热至斑点清晰,在日光下检视,无患子及其在制剂中的成分分离效果良好,色谱斑点清晰,耐用性好。结论该法操作简便可靠,分离度较好,可作为无患子及其制剂的鉴别方法。
Objective To investigate a method to identificate Wuhuanzi and its preparation . Methods Using TLC for qualitative identification .TLC conditions were investigated under different conditions ,select the appropri‐ate TLC conditions . Results Best TLC conditions ：n‐butanol‐ethyl acetate‐water‐concentrated ammonia （40∶10∶50∶1 ;V∶V∶V∶V ） supernatant as the expansion agent ,opening in the indoor environment and drying out , spraying 5% phosphomolybdic acid in ethanol solution ,heating 105 ℃ to spot clear view in daylight ,separation of Wuhuanzi was satisfactory ,chromatographic spots were clear and good durability . Conclusion The TLC method is simple ,reliable .It also show good separating degree which can be used as the quality control standard and its preparation method .
出处
《蛇志》
2014年第4期370-373,共4页
Journal of Snake
基金
江门市医学卫生科学技术研究项目(编号12A126)
