摘要
目的 了解氟、铝联合暴露对仔鼠生理、神经发育及学习记忆能力的影响。方法 将36只清洁级SD成年妊娠大鼠随机分为9组,分别为对照（自来水）组和氟化钠（100、200 mg/L）、氯化铝（500、1 000 mg/L）单独染毒组及氟化钠＋氯化铝联合染毒组,每组4只。从受孕第0天至仔鼠出生第21天（postnatal day21,PND21,即哺乳期）,母鼠采用自由饮水方式进行染毒;从每窝随机选取12只仔鼠（雌雄各半）继续饲养,自PND22（断乳后）~PND60（成年）期间,采用自由饮水方式进行染毒。观察仔鼠生理发育及神经行为反射指标,采用Morris水迷宫测试PND60仔鼠的学习记忆能力。结果 氟铝联合暴露对仔鼠生理发育、神经反射时间及首次达台时间的影响未见交互作用。与对照组比较,氟铝联合暴露组仔鼠第3、6、9周体重降低,逃避潜伏期延长及穿越平台次数减少,且存在交互作用（F值分别为：8.065、7.521、4.961,P〈0.05）,且均为拮抗作用。结论 在本实验剂量下,妊娠期至成年期高剂量的氟和铝单独暴露及氟铝联合暴露可降低仔鼠空间学习记忆的能力,且氟铝联合暴露对仔鼠空间学习记忆能力的损伤具有拮抗作用。
Objetive To investigate the joint effects of fluoride and aluminum on rat offsprings＇ growth and neurodevelopment and learning and memory abilities. Methods Thirty-six clean grade SD pregnant rats were randomly divided into nine groups, four in each, the control groups （drinking tap water）,fluorine group （100 and 200 mg/L NaF, dissolved in tap water）, aluminum group （500 and 1 000 mg/L AlCl3,dissolved in tap water）, fluoride plus aluminum group. The pregnant rats were given NaF and AlCl3 through drinking water from the 0 day of pregnancy to postnatal day 21（PND21, lactation）, and from each group, twelve offspring rats were randomly selected to continue feeding. The offspring rats were given the same doses of NaF＋AlCl3 as their maternal rats through drinking water from weaning to adult. The rat offsprings＇ physiological development and the neurobehavioral reflection indexes were observed, and the learning and memory ability of the PND60 young rats was tested by the Morris water maze. Results There was no interaction between fluoride and aluminum on neural reflex and physiogenesis time and the time of the first reaching platform. Compared with the control,at the 3rd,6th and 9th week of exposure, the weights of rats exposed to fluoride and aluminum in combination were lower,the escape latency was extended and the times across platform were decreased,with the interaction （F=8.065, 7.521, 4.961, P〈0.05）, and all were antagonism. Conclusion Under the experimental doses, exposure of the higher dose of fluoride and higher dose of fluoride aluminum lonely and fluoride combined with aluminum during uterus and adult period, may reduce the learning and memory ability of rat offsprings. Maybe the joint neurotoxicity of fluoride and aluminum is antagonistic.
出处
《环境与健康杂志》
CAS
CSCD
北大核心
2014年第9期773-776,共4页
Journal of Environment and Health
基金
贵州省科技厅科学技术基金(黔科合J字[2010]2189号)
关键词
氟
铝
神经行为
学习
记忆
Fluorine
Aluminum
Neurobehavior
Learning
Memory
