摘要
为提高兴安百里香繁殖速度,研究了兴安百里香的离体快繁技术。研究结果表明:以带腋芽茎段为外植体,1g·LHgCl2消毒7 min为宜;适宜的诱导愈伤组织培养基为MS+6-BA0.25 mg·L-1+IAA1.0 mg·L-1,适宜的愈伤组织分化培养基为MS+6-BA0.5 mg·L-1+IAA0.5 mg·L-1,适宜的继代增殖培养基为MS+IBA0.5 mg·L-1,适宜的最佳生根培养基为1/2MS+IBA0.5 mg·L-1。
In order to improve the reproduction rate of Thymus dahuricus,the fast in vitro propagationtechnology was studied. The results indicated that the stem segment with buds was the best explant,and the optimum sterilization time was 7 min in1g·L HgCl2; MS ＋ 6- BA0. 25 mg·L^- 1＋ IAA1. 0 mg·L^- 1was the optimum medium for primary culture; the most suitable medium for callus differentiation was MS ＋ 6- BA0. 5 mg·L^- 1＋ IAA0. 5 mg·L^- 1; the most suitable medium for proliferation was MS ＋ IBA0. 5 mg·L^- 1; and 1 /2MS ＋ IBA0. 5 mg·L^- 1was the best medium for root induction.
出处
《中国城市林业》
2014年第6期23-25,共3页
Journal of Chinese Urban Forestry
关键词
兴安百里香
离体繁殖
培养基
Thymus dahuricus
in vitro propagation
medium
