摘要
目的研究与唾液腺腺样囊性癌(SACC)侵袭转移相关的微小RNA(mi RNA),探讨mi R-181a对SACC侵袭和迁移的影响。方法选择1对不同侵袭迁移能力的SACC细胞株(SACC-LM/SACC-83),采用mi RNA芯片技术分析细胞株中mi RNA的表达差异,初步筛选出与SACC侵袭转移相关的mi RNA。再通过过表达或沉默细胞株中mi R-181a的表达,进行细胞划痕和Transwell侵袭实验检测细胞的侵袭、迁移能力。采用SPSS 17.0软件对数据进行统计学处理,以P<0.05为差异具有统计学意义。结果 mi RNA芯片结果显示,高侵袭迁移能力的SACC细胞株mi R-181a表达明显下降。细胞划痕和Transwell实验结果表明,SACC-LM细胞转染mi R-181a mimics后体外侵袭迁移能力明显受到抑制(t=-5.235,P<0.05);SACC-83细胞转染mi R-181a LNA后体外侵袭迁移能力有所提高(t=7.713,P<0.05)。结论不同侵袭迁移能力的SACC细胞株中存在多种mi RNA的差异表达。mi R-181a的表达降低可能导致SACC细胞侵袭迁移能力的增强。
Objective To screen micro RNA（miRNA） related to the cell invasion and metastasis in salivary adenoid cystic carcinoma（SACC） cell lines and investigate the effect of miR-181 a in the cell invasion and migration in SACC. Methods A paired SACC cell lines（SACC-LM / SACC-83） with different cell invasive and migrative ability were chosen. Mi RNA array was performed to analyze the different expression of miRNAs in the paired SACC cell lines and identify miRNAs related to the cell invasion and metastasis. Then SACC-LM and SACC-83 were transfected with miR-181 a mimics and miR-181 a LNA respectively. Wound healing assay and transwell assay were used to observe the changes of the cell migration and invasion ability after transfection. Data were analyzed using the Statistical Package for the Social Science（SPSS）, Version 17.0. For all statistical analyses, P〈0.05 was considered statistically significant. Results Mi RNA array showed that the expression of miR-181 a in SACC-LM cells was lower than SACC-83 cells. Wound healing assay and transwell assay showed that ectopic transfection of the miR-181 a mimics into the SACC-LM cells led to inhibition in the cell invasion and migration（t=-5.235,P〈0.05）. When the SACC-83 cells were treated with miR-181 a LNA, an improvement in the cell invasive and migrative ability was observed（t=7.713,P〈0.05）. Conclusions There are various miRNAs expressing differently in SACC cell lines with divergent invasive and migrative ability. Underexpression of miR-181 a could enhance the cell invasive and migrative ability of SACC cells.
出处
《中华口腔医学研究杂志（电子版）》
CAS
2014年第6期5-9,共5页
Chinese Journal of Stomatological Research(Electronic Edition)
基金
广东省科技计划国际合作项目(1011420600001)
高校基本科研业务费"中山大学青年教师重点培育计划"(11ykzd09)
