摘要
以猪场粪污水厌氧发酵产生的沼液为材料,分别在冬春季和夏秋季对其采用加盖和无盖2种方式贮存,定期检测沼液中大肠菌群数量;通过田间生菜种植试验,定期施用大肠菌群数量(以下均以CFU计)分别为104(WF)、105(LF)、106(MF)和107g-1(HF)的沼液,在沼液施用完毕后第3、10、17和24天检测土壤、生菜表面和生菜体内大肠菌群数量。结果表明:夏秋季大肠菌群存活时间较长,沼液无盖贮存可使大肠菌群数量减少。沼液贮存50 d后,除冬春季无盖贮存的沼液中大肠菌群数量达到GB 8978—2002《污水综合排放标准》中三级排放要求,可以排放以外,其他处理均未达到排放要求。沼液中大肠菌群数量越高,经农田施用后土壤中大肠菌群数量越多,生菜表面和体内大肠菌群数量也越高,但随时间延长大肠菌群数量变化略有不同。检测期内,WF处理农田土壤中大肠菌群数量显著低于其他处理;沼液施毕后第3天,WF处理生菜表面及体内大肠菌群数量均显著小于HF和MF处理;24 d时各处理生菜体内大肠菌群数量均检测不到,达到GB 4789.3—2010《食品安全国家标准食品微生物学检验大肠菌群计数》要求,生菜表面大肠菌群数量对数值小于3.51,但未达GB 4789.3—2010要求。
Biogas slurry produced from anaerobically digested pig waste from an intensive pig farm was used for study. Population of fecal coliforms in the slurry under storage （ covered or open） in winter and spring, and summer and autumn was monitored. And the slurries, different in coliform population [104（Treatment WF）, 105（Treatment LF）, 106（Treat？ment MF） and 107 cfu＆#183;g-1（ Treatment HF） ] were top？dressed separately on lettuce in the field. Samples of soil and lettuce were collected from the field 3, 10, 17 and 24 days after the top？dressing for analysis of population of coliforms in the soil, on the surface of and in the lettuce. Results show that coliforms in the slurry lived longer in SA, and open storage reduced the population of coliforms. After 50 days of storage, all the treatments failed to meet Grade III criteria of the“Integrated Standard for Discharge of Wastewater GB 8978—2002”, except for the treatment of open storage in WS. The more the coliforms that survived in the slurry, the more the coliforms that could be found in the soil, on the surface of and in the lettuce that was top？dressed with the slurry. However, the population of coliforms varied with time. During the monitoring period, Treatment WF under open storage was significantly lower than the others in population of coliforms in the soil. On the 3rd day after the topdressing, Treatment WF was much lower than Treatments HF and MF in number of coliforms on the surface of and in the lettuce, and 24 days after the topdressing, coliforms were no longer detected in the lettuce in all the treatments, which is up to the“National Standard for Food Safety”, however, they could still be found on the surface of the lettuce with logarithmic value of the population being lower than 3.51, which fails to the “National Standard for Food Safety”.
出处
《生态与农村环境学报》
CAS
CSCD
北大核心
2014年第6期806-810,共5页
Journal of Ecology and Rural Environment
基金
农业部公益性行业专项(201203050-1)
江苏省六大人才高峰项目(NY-035)
关键词
大肠菌群
沼液
贮存
田间施用
消长动态
fecal coliform
biogas slurry
storage
top-dressed in the field
dynamic change
