摘要
目的了解杭州地区急性散发性戊型病毒性肝炎患者基因型特点。方法采用病毒核酸提取方法进行基因提取,荧光定量PCR方法筛选,选取代表株进行克隆测序,利用生物信息学软件进行多序列比对排列及构建进化树。结果对78份标本进行检测,经荧光PCR检测16份阳性,阳性率为20.51%。3株代表株经克隆测序,并进行BLAST比较和进化树分析,均属HEVⅣ型,与国内其他地区分离的Ⅳ型毒株核苷酸同源性为84.6%～96.7%。结论杭州地区的急性散发性戊型病毒性肝炎病毒属于Ⅳ型。
Objective To investigate the genotype of hepatitis E virus（ HEV） among patients with acute sporadic hepatitis E in Hangzhou city. Methods Viral nucleic acid extraction method for gene extraction was performed to extract HEV RNA from samples. The real- time fluorescence quantitative PCR was used to screen. The PCR products were cloned and sequenced. The genotype of isolated sequences were analyzed with the molecular biological software. Results Seventy- eight samples from patients with acute sporadic hepatitis E were detected and sixteen were positive for HEV RNA by the real- time fluorescence quantitative PCR. The three representative strains were cloned and sequenced. Phylogenetic tree analysis suggested these 3 isolates belonged to HEV genotype Ⅳ. Compared with human type Ⅳ strains from other areas in China,the homology of nucleotide was84. 6% ～ 96. 7%. Conclusion HEV sequences isolated from patients with acute sporadic hepatitis E in Hangzhou city belong to genotype Ⅳ.
出处
《中国卫生检验杂志》
CAS
2015年第2期223-225,共3页
Chinese Journal of Health Laboratory Technology
基金
浙江省医药卫生科技资助项目(2011KYB078)
