摘要
本文以色谱柱SUPELCOSIL LC-18-DB,33*4.6 mm,3μm,流速3.0 m L/min,柱温25℃,检测波长238nm,以乙腈-0.1%磷酸(50∶50)和0.1%的磷酸作为流动相。建立一种高效液相色谱测定辛伐他汀咀嚼片中辛伐他汀含量的方法,并对此法进行考察。结果表明辛伐他汀在浓度为0.195 2 mg/m L^0.608 1 mg/m L(浓度水平50%-150%)范围内具有良好的线性,平均回收率为99.3%,RSD为0.64%(n=9)。
In order to establish an assay to determinate simvastatin content in simvastatin chewable tablets by HPLC,a SUPELCOSIL LC-18-DB chromatographic column（33 × 4. 6 mm,3 μm） was adopted for methodology development. The chromatographic condition was as follows： flow rate,3. 0 m L / min; column temperature,25 ℃; detection wavelength,238 nm; mobile phase,0. 1% acetonitrile-phosphate（50 ∶ 50） and0. 1% of phosphoric acid. The results showed that the simvastatin concentration was linear between 0. 195 2mg / m L and 0. 608 1 mg / m L. The mean recovery rate was 99. 3% with RSD as 0. 64%（n = 6）. The method is reliable and accurate,which is suitable for quantitative detection of simvastatin content in simvastatin chewable tablets.
出处
《山地农业生物学报》
2014年第6期86-89,共4页
Journal of Mountain Agriculture and Biology
基金
贵州省科技厅农业攻关项目[黔科合NY(2011)3094号]及[筑科合同(2012)4-7号]
