摘要
目的:观察益肾通络方对多囊卵巢综合征(PCOS)模型大鼠黄素化颗粒细胞IRS-2蛋白表达及IL-18、IL-10水平的影响。方法:将30只雌性SPF大鼠随机分为3组,即正常组、PCOS模型组、中药组,每组10只,建立PCOS大鼠模型。模型组及正常组灌服生理盐水,中药组灌服益肾通络方,造模后3组大鼠均取出卵巢,进行心脏采血,通过免疫印迹法检测大鼠卵巢黄素化颗粒细胞IRS-2的蛋白表达,酶联免疫吸附试验法检测大鼠血清IL-18、IL-10水平的变化。结果:PCOS组的IRS-2蛋白表达增强,与正常组比较差异有统计学意义(P<0.01);中药组的IRS-2蛋白表达较PCOS组下降,差异有统计学意义(P<0.05)。PCOS组的IL-18水平升高,与正常组比较差异有统计学意义(P<0.01);中药组IL-18水平显著下降,与PCOS组比较差异有统计学意义(P<0.01)。PCOS组的IL-10水平降低,与正常组比较有统计学意义(P<0.05);中药组的IL-10水平上升,与PCOS组比较有统计学意义(P<0.05)。结论:益肾通络方可以调节PCOS大鼠黄素化颗粒细胞IRS-2蛋白表达。PCOS大鼠黄素化颗粒细胞IRS-2参与PCOS的发病,但是产生这种变化的原因尚不清楚。同时亦证明了益肾通络方对PCOS的发病可能起到双向调节作用。
Objective：To observe the effect of Yishen Tongluo formula on expression of IRS-2 protein in granulosa cells and level of IL-18 and IL-10 of Model Rats of PCOS. Methods： 30 SPF female rats were randomly divided into 3 groups：control group,PCOS model group and Chinese medicine group. Each group had 10 rats. 3 days after the model of PCOS established ,all rats in the 3 groups had their ovaries removed and heart blood collected. Detected the rats ’ protein expression of IRS-2 in granulosa cells by western blot method and level of IL-18 and IL-10 by enzyme-linked immunosorbent assay（ELISA） method. Results：The protein expression of IRS-2 of PCOS model group was significantly higher than that of control group（P＜0.01）, while the protein expression of IRS-2 of Chinese medicine group was lower than that of PCOS group（P＜0.05）;The level of IL-18 of PCOS model group was significantly higher than that of control group（P＜0.01）,while the level of IL-18 of Chinese medicine group was significantly lower than that of PCOS model group（P＜0.01）;The level of IL-10 of PCOS model group was lower than that of control group（P＜0.05）,while the level of IL-10 of Chinese medicine group was higher than that of PCOS model group（P＜0.05）. Conclusion：Yishen Tongluo formula can regulate the expression of IRS-2 protein in granulosa cells of PCOS model rats. The IRS-2 in granulosa cell of PCOS rats was involved in the onset of PCOS,but the reason was clear. It was also proved that Yishen Tongluo formula may had bidirectional regulatory effect on PCOS.
出处
《山东中医药大学学报》
2015年第1期76-78,共3页
Journal of Shandong University of Traditional Chinese Medicine
