摘要
对已建立的PEG突变体库中随机挑选的320株突变体菌株进行表型和致病性筛选,获得表型或致病性显著变化的菌株。将突变菌株及野生菌株接种至PDA培养基,观察生长3d和7d时菌落颜色及形态,测量菌落直径。将表型变化菌株及野生菌株接种于离体富士叶片,测量接种5d后病斑直径。生长速率检测显示有40株生长速率加快,有3株生长速率显著下降。14株突变体菌落颜色发生明显变化,菌落呈黑色或纯白色;15株突变体菌落形状不规则且气生菌丝稀疏;其中7株同时具有两种表型特征。致病性测定获得致病性增强突变体s104和致病性减弱突变体s172。所筛选的320株突变体中,表型异常菌株占6.8%,同时获得两株致病性变化菌株,对进一步揭示Botryosphaeria dothidea的致病机理具有重要价值。
Selecting 320 of mutant strains from established PEG mutant library, phenotype and patho- genicity were screened to obtain phenotype and pathogenicity changed strains. The mutant and wild strains were cultured on PDA medium. Color of colonies were observed after 3 days＇ growth and 7 days＇ growth. Meanwhile, diameter of colonies were measured. Strains of abnormal phenotype and wild type were inoculated on detached Fuji leaves and diameter of lesions were measured after 5 days later . 40 strains showed a faster growth and 3 strains grew slower. 14 strains showed obvious differ- ences in colony color which turned to black or white and 15 strains presented asymmetrical shaped col- onies with sparse aerial hyphae, and seven strains had both phenotypic characteristics. The pathoge- nicity test found a pathogen-enhanced Mutant s104 and pathogen-weakened mutant s172. 6.8% of se- lected 320 strains showed abnormal phenotype. Besides, two strains with different pathogenicity were obtained, which has great value in further study for pathogenisis of Botryosphaeria dothidea.
出处
《西北农业学报》
CAS
CSCD
北大核心
2015年第2期146-150,共5页
Acta Agriculturae Boreali-occidentalis Sinica
基金
公益性行业(农业)科研专项(201103016)
高等学校学科创新引智计划(B07049)
