摘要
目的建立印度獐牙菜指纹图谱,探讨和验证双标线性校正法结合PDA辅助法用于色谱峰定性的可行性。方法采用HPLC建立印度獐牙菜指纹图谱,使用相似度分析和二维聚类分析进行质量评价,并使用双标线性校正结合PDA辅助法进行色谱峰定性。结果 10批次印度獐牙菜药材选定10个色谱峰作为指纹图谱的共有峰,经化学计量学评价,可对其质量进行初步评价;双标线性校正法预测保留时间的精度优于相对保留时间法;双标线性校正法结合PDA辅助法可更好的对色谱峰进行定性。结论与相对保留时间法相比,双标线性校正法预测保留时间的准确度更高,色谱柱的适用范围更广。双标线性校正结合PDA对色谱法进行辅助定性可扩大双标线性校正的使用范围。
OBJECTIVE To establish the HPLC fingerprint of Swertia chirayita,and validate the feasibility of the double standard linear correction method using two reference substances with PDA assistance.METHODS The fingerprint of Swertia chirayita was established by HPLC,and similarity analysis and two-dimensional clustering analysis were used for quality evaluation.Linear calibration using two reference substances with PDA assistance was used for identification of chromatographic peak.RESULTS Ten chromatographic peaks were selected as common peaks in the fingerprint of 10 batches of Swertia chirayita.Preliminary evaluation of the quality was made by chemometrics evaluation.The precision of predicting retention time by linear calibration using two reference substances was superior to the relative retention time method.Linear calibration using two reference substances with PDA assistance could qualify the chromatographic peaks better.CONCLUSION Linear calibration using two reference substances is more accurate and suitable for more chromatographic columns than relative retention time method.PDA assistance can enlarge the scope of application of linear calibration using two reference substances.
出处
《中国药学杂志》
CAS
CSCD
北大核心
2015年第4期287-292,共6页
Chinese Pharmaceutical Journal
基金
国家十二五"重大新药创制"专项(2014ZX09304307-002)
国家自然科学基金资助项目(81303214
81173506)
国家食药总局进口药材标准增修订专项(JKYC2012-14)
