摘要
研究银杏叶中总黄酮的最佳提取工艺条件。在对乙醇浓度、固液比与提取时间进行单因素试验的基础上,设计三因素三水平的L9(33)正交试验优化超声辅助乙醇提取银杏叶总黄酮的最佳提取条件。结果表明:银杏叶中总黄酮的最佳工艺条件为:乙醇浓度60%、固液比1∶50(m∶V)与提取时间30min。通过差异性分析可知,乙醇浓度对总黄酮的提取率影响最大(P<0.01),其次为固液比(P<0.05),提取时间对其提取率影响最小(P>0.05)。在最佳工艺条件下,银杏叶醇提物中的总黄酮提取量为8.075 mg/g,提取率为80.75%。
Objective to optimize the technology for extracting process of total flavonoids fromGinkgo bilobaleaves.Methods Based on the single factor experiments for ethanol concentration,solid-liquid ratio and extracting time,orthogonal experiment L9（33）was designed to optimize the ethanol extracting conditions of total flavonoids by ultrasonic-assisted method.Results the optimal technology for extracting conditions of total flavonoids was as followed：with 60%ethanol concentration,1∶50（m∶V）solid-liquid ratio and 30 min of extracting time.Conclusion According to the results of variance analysis,ethanol concentration（P〈0.01）had the greatest influence on yield of total flavonoids,followed by solid-liquid ratio（P〈0.05）,extracting time was the minimal influence（P〉0.05）.Under these conditions,the yield of total flavonoids was 8.075mg/g and the extracting rate was 80.75%.
出处
《食品与机械》
CSCD
北大核心
2015年第1期167-170,共4页
Food and Machinery
基金
"十二五"国家支撑计划项目(编号:2012BAD38B09)
上海海洋大学科技发展专项基金(编号:A2-0209-14-200061)
2014年上海市大学生创新活动计划项目(编号:A1-2035-14-0007-11-3)
关键词
超声波辅助法
银杏叶
总黄酮
ultrasonic-assisted method
Ginkgo biloba leaves
total
