摘要
目的:探讨磷酸化表皮生长因子受体(p-EGFR)及其下游信号磷酸化蛋白激酶Akt(p-Akt)在食管鳞癌组织中的表达及其相关性。方法:采用免疫组化法检测50例食管鳞癌组织和50例相应癌旁正常食管黏膜组织中p-EGFR和p-Akt蛋白的表达,并分析二者与食管鳞癌患者临床病理特征的关系以及二者在食管鳞癌组织中表达的相关性。结果:食管癌组织中p-EGFR和p-Akt蛋白表达水平均高于癌旁正常食管黏膜组织(χ2配对=9.818和33.390,P<0.05)。p-EGFR和p-Akt蛋白的表达与食管鳞癌的分化程度(χ2=18.083和11.049,P<0.05)、淋巴结转移(χ2=7.284和5.817,P<0.05)有关,与患者的性别、肿瘤直径及TNM分期无关(P>0.05)。此外,p-EGFR和p-Akt蛋白在食管鳞癌中的表达呈正关联(rP=0.501,P<0.001)。结论:p-EGFR和p-Akt蛋白的高表达可能与食管鳞癌的恶性程度密切相关。
Aim： To investigate the expressions of phosphorylated epidermal growth factor receptor（ p-EGFR） and its downstream signaling phosphorylated Akt（ p-Akt） in esophageal squamous cell carcinoma（ ESCC） tissue and their correlation.Methods： Immunohistochemistry was utilized to detect the expressions of p-EGFR and p-Akt proteins in 50 cases of ESCC tissue and matched normal esophageal epithelial tissue,and their correlation in ESCC tissue was investigated.Results： The expressions of p-EGFR and p-Akt proteins in ESCC tissue were significantly higher than those in normal esophageal epithelial tissue（ χ^2paired= 9.818 and 33.390,P〈 0.05）.Additionally,the expressions of p-EGFR and p-Akt proteins in ESCC tissue with poor differentiation and lymph node metastasis were markedly higher than those in ESCC tissue with high differentiation（ χ^2= 18.083 and 11.049,P〈 0.05） and without lymph node metastasis（ χ^2= 7.284 and 5.817,P〈 0.05）,however,the ex-pressions of p-EGFR and p-Akt proteins were not related to the patients＇ gender,tumor size or TNM staging（ P〈 0.05）.Moreover,the expressions of p-EGFR and p-Akt proteins in ESCC tissue displayed positive correlation（ r P= 0.501,P〈 0.001）.Conclusion： p-EGFR and p-Akt proteins at high level may be closely associated with the malignant degree of ESCC.
出处
《郑州大学学报（医学版）》
CAS
北大核心
2015年第1期5-8,共4页
Journal of Zhengzhou University(Medical Sciences)
基金
河南省科技攻关基金资助项目112102310090
关键词
食管鳞癌
表皮生长因子受体
AKT
磷酸化
esophageal squamous cell carcinoma
epidermal growth factor receptor
Akt
phosphorylation
