摘要
目的:观察大鼠二次脑损伤后脑组织SOD及MDA的变化,进一步探讨过氧化反应在二次脑损伤机制中的作用。方法:90只雄性SD大鼠随机分为正常对照组(A组)、弥漫性脑损伤组(B组)和二次脑损伤组(C组),建模成功后,分别于伤后1 h、3 h、6h、12 h、24 h、48 h处死动物,取额叶组织匀浆,分别测定大鼠脑内SOD及MDA的含量。结果:伤后1h开始,B、C组中SOD含量呈现先上升后下降的趋势,并随时间呈持续下降趋势直至伤后24 h,C组较B组下降程度更加明显(P<0.05);伤后3 h,B、C组中SOD含量均增高,B组增高程度和速率均高于C组(P<0.05)。伤后1 h开始,B、C组MDA含量呈升高趋势,并于24小时达峰值,C组升高趋势较B组更显著,伤后6 h、24 h、48 h,C组与B组之间相比统计学差异显著(P<0.05)。结论:创伤性脑损伤及二次脑损伤后,脑组织内过氧化反应明显加重,SOD及MDA均出现明显变化,二次脑损伤的过氧化反应较脑损伤组更加严重且持续时间更长,且MDA较SOD的变化具有滞后性。
Objective： Testing the changes of superoxide dismutase（SOD） and malondialdehyde （MDA） in rats with diffuse brain injury （DBI） or secondary brain insult （SBI）, to analyze the effects and significance of peroxidation in development of rats SBI. Methods： 90 male SD rats were randomly divided into normal control group （group A）, DBI group （group B） and SBI group （group C）. After the success of the modeling, the rats were put to death, and the frontal tissue homogenate were taken after injury 1 h, 3 h, 6 h, 12 h, 24 h, and 48 h respectively, to determine the content of SOD and MDA. Results： From the first 1 h after injury, SOD content in B and C groups presents the downward trend after rising first, and the declining trend over time until 24 h after injury, the degree of decline in group C is more obvious compared with that in group B （P〈 0.05）; 3 h after injury, SOD content in B and C groups were higher, the higher degree and the rate of B group were higher than in group C （P〈0.05）. From the first 1 h after injury, MDA content in groups B and showed a trend of increase, and in 24 hours to spike, group C increased more significantly than group B, at 6 h, 24 h, and 48 h after injury, the differences between Group B and Group C were statistically significant （P〈0.05）. Conclusion： After DBI or SBI in rats, peroxidation in- crease obviously, SOD and MDA change ap- parently, peroxidation in SBI is more serious than that in DBI and last longer, and the changes of MDA are later than those of SOD.
出处
《现代生物医学进展》
CAS
2015年第5期812-814,821,共4页
Progress in Modern Biomedicine
基金
南京军区医药卫生基金(12M015A)
福建省自然科学基金项目(2014J01425)
关键词
弥漫性脑损伤
二次脑损伤
超氧化物歧化酶
丙二醛
Diffuse brain injury
SeCondary brain insult
Superoxide dismutase
Malondialdehyde
