摘要
为探索不同胁迫对沟叶结缕草（Zoysia matrella[L.]Merr.）愈伤组织生长及再生的影响,以继代了9年的胚性愈伤组织为材料,以MS（murashige and skoog）为基本培养基添加不同浓度的铜（Cu2＋）,研究其对继代培养中愈伤组织的直径、鲜重增长率和再生培养中的植株再生率,以及愈伤组织及再生植株根和叶的过氧化氢酶（CAT）、过氧化物酶（POD）、超氧化物歧化酶（SOD）和抗坏血酸过氧化物酶（APX）活性、铜含量、叶绿素含量、丙二醛（MDA）含量的影响。结果表明,在铜浓度为10.0μM时,愈伤组织直径、鲜重增长率、植株再生率及叶绿素含量均达最大值;愈伤组织可生长和再生的铜的临界浓度分别在300.0~500.0μM、50.0~100.0μM间。经长期处理（12周）的愈伤组织和24周再生的植株叶片和根系中,4种抗氧化活性酶活性和MDA含量在10.0μM下均为最低;经短期处理（10 d）的愈伤组织,SOD、POD、APX的活性及MDA含量持续上升,而CAT先上升后下降。愈伤组织、再生植株叶片和根系中铜含量随培养基中铜浓度的升高而增加。本试验为进一步筛选耐铜突变体提供了试验依据。
The embryonic callus of Zoysia matrella subcultured for 9 years was used to study its responses to copper stress during callus growth and plant regeneration. The effects of different concentrations of copper on callus diameter,growth rate of fresh weight and regeneration rate,the activities of antioxidant enzymes（ CAT,POD,SOD and APX）,the contents of copper,MDA in the callus,the leaf and root of the plantlets in the courses of callus growth and plant regeneration were evaluated. It was showed that the callus diameter,growth rate of fresh weight,regeneration rate and the content of leaf chlorophyll reached the maximum when copper at 10. 0 μM,and the critical concentrations of copper for callus growth and plant regeneration were between 300. 0 to 500. 0 μM and 50. 0 to 100. 0 μM,respectively. After long-term callus growth maintenance（ 12 weeks） or plant regeneration（ 24 weeks）,the activities of the 4 kinds of antioxidant enzymes,the contents of MDA at 10. 0 μM of copper showed the lowest levels; while after short-term（ 10days） callus growth culture,the enzyme activities except CAT increased as the rising of the copper concentrations. The contents of copper in the callus, leaf and root of the regenerated plantlets increased with the rising of copper concentrations on media.
出处
《核农学报》
CAS
CSCD
北大核心
2015年第1期161-168,共8页
Journal of Nuclear Agricultural Sciences
基金
国家自然科学基金项目(30771522)
关键词
沟叶结缕草
愈伤组织
铜胁迫
生理特性
Zoysia matrella
callus
copper stress
physiological characteristics
