摘要
为研究孔雀石绿(MG)残留在亲鱼及其子代之间的传递作用以及鱼卵阶段违禁使用孔雀石绿的溯源分析,通过硼氢化钾将孔雀石绿还原成无色孔雀石绿,采用高效液相色谱-荧光检测法检测孔雀石绿及其代谢产物的总量。以浓度为0.5mg/L的孔雀石绿浸泡鲫亲鱼4h,分别跟踪检测雌雄亲鱼肌肉、内脏团以及生殖组织中药物残留变化。取第30天带有MG残留的雌雄亲鱼与空白亲鱼交叉繁殖,检测子代受精卵与鱼苗中药物残留情况,并与空白鱼卵浸泡0.15mg/L孔雀石绿溶液1h后的残留情况作比较。结果显示,各组织中孔雀石绿的残留分布情况在雌雄亲鱼之间无显著性差异;不同组织中残留浓度大小:内脏团>肌肉>精巢/卵巢,内脏团和肌肉中孔雀石绿残留浓度逐渐下降,精巢/卵巢从0.25h检测到药物残留,之后呈先增加后缓慢下降的趋势。污染孔雀石绿的亲鱼所产的受精卵中,药物残留初始浓度较低,分别为5.1μg/kg±0.8μg/kg、23.8μg/kg±1.3μg/kg、29.5μg/kg±1.1μg/kg,均缓慢下降。而空白受精卵浸泡孔雀石绿溶液后的初始浓度为1 265.8μg/kg±21.1μg/kg,在出苗前保持在较高的浓度,在鱼苗完全孵出后(第5天)残留浓度大幅度下降为163.4μg/kg±7.9μg/kg。因此,亲鱼体内残留的孔雀石绿及其代谢产物残留能够传递到子代,并可根据鱼卵中药物残留浓度变化的差异,区别判断受精卵污染孔雀石绿的来源途径,为养殖环节的孔雀石绿残留溯源分析提供参考。
In order to study the parent-child pass effect of malachite green residues and traceability analysis on MG used during the roe stage,parent fish were treated at 0.5 mg/L of MG dipping for 1 hour,and HPLC-FLD was used to detect MG residues in the tissues of muscle,visceral mass and ovary or testis,re-spectively.In the 30th day,polluted parent fish and blank ones were cross-breeded,and then MG residues in fertilized eggs and fry were detected and compared to those blank fertilized eggs which were treated at 0.15 mg/L of MG dipping for 1 hour.The results showed that the MG residues and distribution in differ-ent tissues of male and female parent fish had no significant difference;The residual concentrations from high to low were in visceral mass,muscle,ovary or testis.The MG residue concentration of visceral mass and muscle gradually decreases,and MG in ovarian or testis was detectible from 0.25 h,decreasing slowly. MG residues could be passed from parent fish to offspring,however,the medicine residues concentrations were in a low levels for 5.1,23.8,29.5μg/kg,respectively.However,the initial concentration of MG af-ter blank fertilized eggs dipping in MG solution was 1 2 6 5 .8μg/kg and it remained at a high level and sharply fell to 1 6 3 .4μg/kg after coming out from membrane in the 5 th day.According to the different concentrations of MG residues in fertilized eggs,the ways to use MG during roe stage would be found out, which could provide theoretical reference for traceability analysis of MG residues during breed tache.
出处
《动物医学进展》
北大核心
2015年第3期68-72,共5页
Progress In Veterinary Medicine
基金
公益性行业(农业)科研专项经费项目(201203085)
上海市科技兴农重点攻关项目(沪农科攻字(2011)第4-8号)
鱼虾用疫苗与药物研究开发863项目(2011AA10A216)
国家水产种质资源平台运行服务项目
上海高校水产一级重点学科及上海高校知识服务平台(ZF1206)
