摘要
目的:通过检测初发Graves病(GD)患者的IFN-γ、IL-6、IL-17和TGF-β1等4种细胞因子水平,研究Graves病的发病机制与细胞因子的关系。方法:对23例初发Graves病(GD组)的患者和23名健康对照者(NC组)的血清分别用ELISA法检测IFN-γ、IL-6、IL-17和TGF-β1浓度,用电化学发光法检测FT3、FT4和TSH水平,用放射受体分析法测定Tr Ab水平。结果:GD组与NC组之间年龄、性别均无统计学差异(t=0.334 8,P>0.05;χ2=0.410 7,P>0.05)。GD组IFN-γ、IL-6、IL-17和TGF-β1的浓度显著高于NC组,差异有统计学意义(P均<0.05);4种细胞因子与FT3、FT4均呈正相关(r=值依次为:0.324 6、0.453 2、0.431 0、0.463 8;0.413 2、0.441 5、0.436 2、0.467 1;P均<0.05);与TSH均呈负相关(r=值依次为-0.458 8、-0.482 8、-0.445 1、-0.493 0;P均<0.05)。结论:IFN-γ、IL-6、IL-17和TGF-β1等4种细胞因子在初发GD中均高表达;可能共同参与了GD的发病过程,可作为GD免疫功能紊乱的评价指标。
Objective：To explore the role of cytokines in the pathogenesis of Graves＇disease （ GD）, by detecting the levels of IFN-γ, IL-6, IL-17 and TGF-β1 in GD patients who were newly diagnosed. Methods： A total of 23 patients with new onset GD and 23 gender- and age-matched healthy controls were examined. The levels of serum IFN-γ, IL-6, IL-17 and TGF-β1 were measured by ELISA, FIB,FT4 and TSH levels were determined by ECL IA;TrAb levels were tested by RRA. Results： There were no significant difference among GD and NC group in sex and age match（t =0. 334 8 ,P〉0.05 ;X2 =0. 410 7 ,P〉0. 05）. The levels of serum IFN-3,,IL- 6, IL-17 and TGF-~I in the GD group were significantly higher than the control group （P〈0. 05 ） . Correlation analysis revealed that IFN-γ,IL-6,IL-17 and TGF-β1 were positively correlated with FT3 ,FT4（r=0. 324 6,0. 453 2,0. 431 0,0. 463 8 ;0. 413 2,0. 441 5, 0.436 2,0.467 I; P〈0.05）. Conclusion： IFN-γ, IL-6, IL-17 and TGF-β1 are highly expressed in the newly diagnosed GD patients. They play an important role in the pathogenesis of GD, and provide helpful evaluation indices of immune dysfunction to Graves disease.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
2015年第2期253-256,共4页
Chinese Journal of Immunology
