摘要
目的观察热化联合对肺癌患者A549细胞生长的影响及机制探讨。方法对A549细胞分别进行单独热疗、单独化疗,热化联合干预及热化联合并SP600125干预,同时选取未做任何处理的A549细胞作为对照组。观察各组细胞增殖率、细胞侵袭力的变化。同时采用蛋白免疫印记法(Western Bolt)检测JNK磷酸化以及热休克蛋白70(HSP70)的表达。结果热化联合组的A549细胞增值率明显低于单独热疗、单独化疗和热化联合并SP600125组(P<0.05)。热化联合组JNK磷酸化表达明显高于对照组及单独化疗组(P<0.05),热化联合组HSP70表达明显低于单独热疗组(P<0.05)。热化联合干预下,p-JNK表达水平出现上升,与对照组、单独热疗组和单独化疗组相比,差异均具有统计学意义(P<0.05);热化联合并SP600125组的p-JNK的表达水平较热化联合组显著下降(P<0.05)。结论热化联合抑制A549细胞增殖的效果优于单独热疗或单独化疗,作用机制可能与激活JNK信号通路或抑制HSP70表达有关。
Objective To investigate the effect of thermalization on A549 cells growth in patients with lung cancer and its mechanism. Methods A549 cells were given thermotherapy alone（group A）, chemotherapy alone（group B）, and thermotherapy combined with chemotherapy（group C）, thermotherapy combined with chemotherapy and SP600125 intervention（group D）. Untreated A549 cells were selected as the control group. The changes of cell invasion, proliferation rate of the cells in each group were observed. Phosphorylated JNK and expression of heat shock protein 70（HSP70） were detected by Western blot. Results A549 cell proliferation rate of group C was significantly lower than that of group A, group B and group D（P〈0.05）. The expression of group C was significantly higher than that of control group and group B（P〈0.05）, and the expression of HSP70 in group C was significantly lower than that in group A（P〈0.05）. In group C, the expression level of p-JNK increased, compared with the control group, group A and group B, with statistically significant difference（P〈0.05）. In group D, the expression level of p-JNK decreased significantly compared with group C（P〈0.05）. Conclusion Thermotherapy combined with chemotherapy had better effects in inhibiting proliferation of A549 cells than thermotherapy or chemotherapy alone. The mechanism may be related to the activation of JNK signal pathway or inhibiting expression of HSP70.
出处
《海南医学》
CAS
2015年第3期326-328,共3页
Hainan Medical Journal
