摘要
目的观察疏肝理气、化湿活血法对胰岛素抵抗3T3-L1脂肪细胞FGF-21水平及其受体FGFR1及FGFR2表达的影响。方法采用1μmol/L地塞米松诱导脂肪细胞胰岛素抵抗。将细胞分为正常对照组、模型对照组、吡格列酮组及中药高、低剂量组。加入含药血清处理后,分别于24h、48h采用葡萄糖氧化酶法检测各孔细胞上清液葡萄糖浓度,采用酶联免疫吸附法检测上清液中FGF-21浓度,培养后48h采用荧光定量PCR法检测细胞FGFR1及FGFR2基因表达水平。结果中药高、低剂量及吡格列酮均可增加胰岛素抵抗3T3-L1脂肪细胞的葡萄糖消耗量,同时降低其FGF-21水平,与相同时间段模型对照组比较均具有统计学意义(P<0.01);吡格列酮可上调胰岛素抵抗3T3-L1脂肪细胞的FGFR1、FGFR2 m RNA表达量(与模型对照组比较P<0.05或P<0.01),中药仅高剂量组可上调其FGFR2 m RNA相对表达量(与模型对照组比较P<0.05)。结论疏肝理气、化湿活血法可改善3T3-L1脂肪细胞胰岛素抵抗水平,其机制可能与FGF信号通路有关。
Objective To investigate the effects of ＂soothing Liver-Qi, resolving dampness and blood stasis＂ on FGF-21 and FGFR1 and FGFR2 levels in 3T3-L1 adipocytes. Methods Insulin-resistant model of 3T3-L1 adipocytes were induced by 1μmol/L dexamethasoneo Then divided into 5 groups： normal control group, model control group Pioglitazone group and Chinese herb with high and low-dose groups. After 24h and 48h cultured with medicated serum, glucose concentration in the culture medium was checked with Glucose Oxidase Method, FGF-21 levels were tested by Enzyme Linked Immunosorbent Assay, and FGFR1/FGFR2 gene expression was measured with Fluorescence Quantitative PCR Method after culturing 48h. Results Glucose consumption was improved, and FGF-21 level was reduced, in insulin-resistant 3T3-L1 adipocytes by Chinese herbs and Pioglitazone （compared with model control group P〈0.01 ）; FGFR1 and FGFR2 mRNA expression in insulin-resistant 3T3-L1 adipocytes were both up- regulated by Pioglitazone （compared with model control group P〈0.05 or P〈0.01 ）, only FGFR2 mRNA expression was up-regulated by high-dose Chinese herbs with a efficiency of ＂soothing Liver-Qi, resolving dampness and blood stasis＂. Conclusion The therapy of ＂soothing Liver-Qi, resolving dampness and blood stasis＂ can alleviate insulin-resistant in 3T3-L1 adipocytes, its mechanism may relate to FGF signal pathway.
出处
《云南中医学院学报》
2015年第1期17-22,共6页
Journal of Yunnan University of Traditional Chinese Medicine
