摘要
背景:临床上使用自体髂骨移植方法修复牙槽嵴裂,常常不能获得稳定的治疗效果,移植骨吸收率因人而异,受到许多因素的影响。目的:观察骨髓间充质干细胞膜片复合自体髂骨松质骨块移植修复牙槽嵴裂术后的骨吸收情况。方法:制备实验犬双侧牙槽嵴裂模型,采用自体对照的办法,在实验犬上颌骨两侧的裂隙分别植入骨髓间充质干细胞膜片复合犬自体髂骨骨块(实验组)和单纯犬自体髂骨骨块(对照组)。通过颌骨CT及micro CT对植骨区的骨吸收率、骨体积分数、骨小梁厚度、骨小梁分离度、骨密度进行比较。结果与结论:术后3,6个月时实验组的骨吸收率明显低于对照组,差异有非常显著性意义(P<0.01);术后6个月实验组骨体积分数、骨密度均高于对照组,差异有显著性意义(P<0.05),实验组骨小梁分离度低于对照组,差异有显著性意义(P<0.05),而骨小梁厚度两组比较差异无显著性意义(P>0.05)。结果表明骨髓间充质干细胞膜片复合髂骨骨移植可减少植骨术后骨吸收率,同时可以促进新骨的生成。
BACKGROUND： Autogenous lilac bone graft for repair of alveolar cleft often cannot achieve a stable therapeutic effect, and the graft resorption rate varies with each individual that is influenced by many factors. OBJECTIVE： To observe the bone resorption after bone marrow mesenchymal stem cell sheet transplantation with autogenous lilac cancellous bone for alveolar cleft repair. METHODS： Bilateral alveolar cleft models were prepared in dogs. Using the self-control method, bone marrow mesenchymal stem cell sheet combined with autogenous iliac bone （experimental group） and autogenous lilac bone alone （control group） were respectively implanted into the bilateral alveolar clefts. Mandible CT and microCT were used to compare the bone resorption rate, bone volume fraction, trabecular thickness, trabecular separation, and bone mineral density. RESULTS AND CONCLUSION： The bone resorption rate was significantly lower in the experimental group than the control group at 3 and 6 postoperative months （P 〈 0.01）; while, the bone volume fracture and bone mineral density of the experimental group were significantly higher than those in the control group at postoperative 6 months （P 〈 0.05）, but the trabecular separation of the experimental group was significantly lower than that in the control group （P 〈 0.05）. In addition, there was no difference in the trabecular thickness between the two groups （P 〉 0.05）. These findings indicate that bone marrow mesenchymal stem cell sheet transplantation combined with autogenous iliac bone graft can reduce bone resorption rate and meanwhile, promote new bone formation.
出处
《中国组织工程研究》
CAS
CSCD
2014年第50期8108-8112,共5页
Chinese Journal of Tissue Engineering Research
基金
河北省科技支撑计划项目(1213078ZD)
项目名称:单侧完全性唇腭裂患者上颌骨矫正后稳定性分析及治疗程序研究~~
