摘要
目的研究不同结构大豆皂甙(SS-A1、SS-A2、SS-I、SG-A和SG-B)拮抗棕榈酸致原代培养小鼠肝细胞氧化应激的活性。方法检测棕榈酸(0.05mmol/L)单独处理或与不同结构大豆皂甙(10μg/ml)共同处理原代培养小鼠肝细胞16h后细胞中超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)、丙二醛(MDA)和甘油三酯(TG)含量以及细胞和线粒体中活性氧(ROS)产生量。结果与对照组相比,棕榈酸处理细胞中SOD、GSH-Px活性显著降低(P<0.05);MDA、TG含量显著升高(P<0.05);细胞和线粒体中ROS均显著升高(P<0.05)。与棕榈酸单独处理组相比,大豆皂甙与棕榈酸共同孵育后能显著增高SOD、GSH-Px活性(P<0.05);降低MDA、TG和ROS含量(P<0.05)。SS-A1、SS-A2处理组与SG-A处理组相比,细胞中SOD、GSH-Px活性显著升高(P<0.05);MDA、ROS含量显著降低(P<0.05)。SS-I处理组与SG-B组相比,细胞中SOD、GSH-Px活性显著升高(P<0.05);ROS含量显著降低(P<0.05)。结论大豆皂甙对棕榈酸引起的小鼠原代培养肝细胞氧化应激具有拮抗活性,结构中含糖侧链的SS-A1、SS-A2和SS-I分别比皂甙元(SG-A和SG-B)具有更强的抗氧化活性。
Objective To investigate the relationship between the structure and activity in protection of soyasaponins against palmitic acid （PA）-indueed oxidative stress in primary mouse hepatocytes. Methods The primary mouse hepatocytes were treated with 0.05 mmol/L PA in the presence or absence of soyasaponins （10μg/ml） for 16h. The activities of superoxide dismutase （SOD）, glutathione peroxidase （GSH-Px）, the contents of malondialdehyde （MDA）, triglyeeride （TG） and reactive oxygen species （ROS） were determined. Results PA treatment significantly lowered cellular SOD and GSH-Px activities （P〈0.05）, increased the contents of MDA and TG （P〈0.05） and the production of ROS in mitochondria was elevated （P〈0.05）. When compared to the treatment of PA alone, the combined treatment of soyasaponins and PA significantly increased the activities of SOD and GSH-Px （P〈0.05） and decreased the contents of MDA, TG and ROS （P〈0.05）. It was found that soyasaponin-A1 or A2 significantly increased the cellular activities of SOD and GSH-Px （P〈0.05） and decreased the contents of MDA and ROS as compared with soyasapogenol-A （P〈0.05）. Similarly, soyasaponin-I significantly increased activities of cellular SOD and GSH-Px （P〈0.05） and decreased the content of ROS as compared with soyasapogenol-B （P〈0.05）. Conclusion Soyasaponins possess antioxidant activity against PA-induced oxidative stress in primary mouse hepatocytes. Soyasaponin-A1, A2 and l are stronger than their corresponding soyasapogenols （soyasapogenol-A and B） in antioxidant activity, probably due to the sugar moieties presented in their chemical structures.
出处
《营养学报》
CAS
CSCD
北大核心
2014年第6期570-576,I0005,共8页
Acta Nutrimenta Sinica
基金
supported financially by grants from National Natural Science Foundation (No. 81102130)
Medical Scientific Research Foundation of Guangdong Province(No. A2011357)
Southern Medical University Undergraduate Out-of-Class Scientific Research Program (No. 2011kw017)
关键词
大豆皂甙
棕榈酸
氧化应激
原代培养小鼠肝细胞
soyasaponin
palmitic acid
oxidative stress
primary mouse hepatocytesAbstract
