微沟槽形貌对人牙龈成纤维细胞整联蛋白intergrin α_5、β_1表达的影响被引量：1

Effects of microgroove surface morphology on the expression of intergrin α_5 and intergrin β_1 in human gingival fibroblasts

下载PDF

导出

摘要目的:研究微沟槽形貌对人牙龈成纤维细胞(HGF)整联蛋白intergrinα5、integrnβ1表达的影响。方法:用光刻技术制作沟槽宽度分别为15、30、60μm,沟槽深度分别为5、10μm(沟槽间隔分别等于各相应宽度)的微沟槽形貌,分别命名为T15/5、T15/10、T30/5、T30/10、T60/5、T60/10,光滑钛(T0)表面作为对照组;分别测量各组材料表面的微沟槽形貌及亲水性,用Real time-PCR和Western blotting检测HGF在各组材料表面培养3 d时的intergrinα5和β1mRNA及蛋白的表达水平。结果:T60/5表面亲水性最好,T15/10疏水性最大并明显大于光滑组T0(P<0.05);intergrinα5mRNA表达水平以T60/10组最高,而T15/10时最低,各组间两两相比P<0.05;沟槽宽度较小时(T15、T30),若深度增加则可减少intergrinα5mRNA的表达,而沟槽宽度较大时(T60),若深度增加则可促进intergrinα5mRNA的表达;intergrinβ1mRNA的表达水平以T60/10组最高,与其他各组相比P<0.05;其余3组间比较均P>0.05,intergrinα5、intergrinβ1蛋白的表达趋势与其mRNA的表达基本一致。结论:intergrinα5和β1表达随着沟槽宽度增大而增加,深度对intergrinα5表达的影响与沟槽的宽度大小相关,沟槽宽度和深度对intergrinβ1表达的影响程度低于intergrinα5。 AIM：To investigate the effects of microgroove surface morphology on the expression of intergrinα5 and intergrin β1 in human gingival fibroblasts（HGFs）.METHODS：Microgroove titanium surface was fabricated by photolithography with parallel grooves：15 μm,30 μm or 60 μm in width and 5 μm or 10 μm in depth.The combi-nations of groove width and depth were hereafter denoted as T15/5,T15/10,T30/5,T30/10,T60/5 and T60/10. Smooth titanium surface（T0）was used as the control.Surface topography and hydrophilicity were detected.HGFs were cultured on the microgroove surfaces for 3 days.Intergrin α5 and intergrin β1 expression was examined by Real-time PCR and western blotting.RESULTS：T60/5 microgroove showed the highest surface hydrophilicity.However, T15/10 had the highest surface hydrophobicity,even higher than T0 （P〈0.05 ）.T60/10 group showed the highest expression of intergrin α5 and intergrin β1 mRNA,and in T15/10 group the lowest （P〈0.05）.In groups with lower

作者赖颖真林珊陈江

机构地区厦门医学高等专科学校口腔医学系福建医科大学附属第一医院口腔科福建医科大学附属口腔医院种植科

出处《牙体牙髓牙周病学杂志》 CAS 2015年第1期8-14,共7页 Chinese Journal of Conservative Dentistry

关键词种植体微沟槽人牙龈成纤维细胞整联蛋白 implant microgroove human gingival fibroblasts intergrin

分类号 R780.2 [医药卫生—口腔医学]

引文网络
相关文献

参考文献15

1Rompen E, Domken O, Degidi M, et al. The effect of material characteristics, of surface topography and of implant components and connections on soft tissue integration: a literature review [J]. Clin Oral Implants Res, 2006,17 (2) :55 -67.
2Lai Y, Chen J, Zhang T, et al. Effect of 3D microgroove surface topography on plasma and cellular fibronectin of human gingival fibroblasts[ J]. J Dent, 2013,41 ( 11 ) : 1109 - 1121.
3Geiger B, Spatz JP, Bershadsky AD. Environmental sensing through focal adhesions[J]. Nat Rev Mol Cell Biol, 2009,10 (1) :21 -33.
4Geiger B, Yamada KM. Molecular architecture and function ofmatrix adhesions[J]. Review. Cold Spring Harb Perspect Biol, 2011,3(5) :1 - 16.
5Schwartz MA. Integrins and extracellular matrix in mechano- transduction[ J]. Cold Spring Harb Perspect Biol , 2010,2(12) : 1 -11.
6Friedland JC, Lee MH, Boettiger D. Mechanically activated in- tegrin switch controls alpha5betal function[ J]. Science, 2009, 323(5914) :642 -644.
7Biggs MJ, Riehards RG, Wilkinson CD, et al. Focal adhesion interactions with topographical structures : a novel method for im- muno-SEM labelling of focal adhesions in S-phase ceils[J]. J Microsc, 2008,231 ( Pt 1 ) :28 - 37.
8Hannafin JA, Atria EA, Henshaw R, et al. Effect of cyclic strain and plating matrix on cell proliferation and integrin expres- sion by ligament fibmblasts [J]. J Orthop Res, 2006,24 ( 2 ) : 149 - 158.
9Boleato-Bellemin AL, Elkaim R, Abehsera A, et al. Expression of mRNAs encoding for alpha and beta integrin subunits, MMPs, and TIMPs in stretched human periodontal ligament and gingival fibroblasts[J]. J Dent Res, 2000,79 ( 9 ) : 1712 - 1716.
10Henshaw DR, Atria E, Bhargava M, et al. Canine ACL fibro- blast integrin expression and cell alignment in response to cyclic tensile strain in three- dimensional collagen gels [J].J Orthop Res, 2006,24 ( 3 ) :481 - 490.