摘要
目的：探究青春期低剂量氰戊菊酯暴露对小鼠行为的影响。方法对出生后第28天的48只ICR小鼠雌雄各半分别进行不同浓度氰戊菊酯（0、0．02、0．20、2．00 mg/kg ）灌胃染毒。出生后第28~56天。在出生后第9周,雌雄小鼠分别进行相关行为学实验。通过黑白巷实验、旷场实验检测小鼠的探索行为和焦虑相关行为,采用Morris水迷宫实验检测小鼠的空间学习和记忆相关行为。结果各组小鼠在旷场实验中的行为差异无统计学意义。在黑白巷实验中,与0 mg/kg组雄性小鼠比较,0．02 mg/kg组和2．00 mg/kg组雄性小鼠跨过中线的潜伏期明显延长（P=0．006,P=0．039）。在Morris水迷宫的定位航行实验中,与0 mg/kg 组雌鼠比较,0．02 mg/kg组和2．00 mg/kg组的雌鼠登陆平台的潜伏期明显延长（P=0．011,P=0．014）；与0 mg/kg组雌鼠比较,0．20 mg/kg 组的雌鼠搜寻平台的距离明显增加（ P =0．003）；而雄性小鼠各组指标之间差异无统计学意义。在Morris水迷宫的空间探索实验中,与0 mg/kg组比较,0．20 mg/kg组雌鼠的目标象限时间比降低（P=0．046）；而雄鼠各组指标之间的差异无统计学意义。结论青春期低剂量的氰戊菊酯暴露能够影响小鼠的多种行为,可能影响小鼠青春期的神经行为发育。
Objective To explore the effects of low dose of fenvalerate exposure during puberty on behaviors in mice. Methods Twenty four male and twenty four female ICR mice in postnatal day 28 were randomly divided into four groups respectively： the control group （0 mg/kg） and fenvalerate group, 0. 02, 0. 20, 2. 00 mg/kg. Mice were treated with corn oil as a vehicle control or fenvalerate by gavage daily from postnatal day 28 to 56 . At the age of 9 weeks, open field, black and white alley, Morris water maze were used to test anxiety, exploratory behavior, spatial learning and memory in mice. Results Exposure to fenvalerate did not alter mice behaviors in open field test. In black and white alley test, pubertal exposure to fenvalerate （0. 02 mg/kg） significantly increased latency in male mice （ P=0. 006 ）;exposure to fenvalerate （ 2. 00 mg/kg ） significantly increased latency in female mice （ P=0. 039）. The results of spatial learning test in Morris water maze showed that pubertal exposure to fenvalerate （0. 20 mg/kg and 2. 00 mg/kg） significantly increased the escape latency of female mice （ P =0. 011, P =0. 014 ）;exposure to fenvalerate （ 0. 20 mg/kg ） significantly extended the escape distance in the female （ P =0. 003 ） . Spatial memory test results showed that the time proportion of objective quadrant was significantly de-creased in female mice treated with 0. 20 mg/kg （ P=0. 046 ） . Exposure to fenvalerate did not alter behaviors of male mice in Morris water maze test. Conclusion Pubertal exposure to low dose fenvalerate disrupts multiple be-haviors and may affect neurobehavioral development in mice.
出处
《安徽医科大学学报》
CAS
北大核心
2015年第2期177-180,共4页
Acta Universitatis Medicinalis Anhui
基金
安徽省自然科学基金(编号:KJ2010A173)
国家自然科学基金(编号:81102155)
安徽医科大学博士科研课题资助(编号:XJ201115)
关键词
青春期
低剂量
氰戊菊酯
行为
学习记忆
puberty low dose fenvalerate behaviors learning and memory
