摘要
目的:研究左乙拉西坦对难治性癫痫患者外周血主穹窿蛋白(MVP)表达的影响,探讨其在难治性癫痫中的作用。方法:收集2012年09月至2013年02月无锡市儿童医院难治性癫痫患者30例,男18例,女12例,年龄(5.2±1.8)岁。这些患者均口服左乙拉西坦超过12个月,分为用药前(B组)、用药6个月(C组)和12个月(D组)3组。选取同期来院体检的健康儿童30例为对照组(A组),男16例,女14例,年龄(5.5±2.9)岁。应用半定量逆转录聚合酶链反应(RT-PCR)方法检测外周血中MVP基因,免疫蛋白印记(Western Blot)方法检测MVP蛋白的表达,结果经统计学处理。结果:空白对照组外周血中有少量MVP表达(mRNA:1.133±0.152、蛋白:0.020±0.008);难治性癫痫患者用药前MVP大量表达(mRNA:1.788±0.097、蛋白:0.209±0.012),与空白对照组比较有显著性差异(P<0.05)。左乙拉西坦治疗组在用药后6个月(mRNA:1.657±0.126,蛋白:0.196±0.012)、12个月(mRNA:1.466±0.199,蛋白:0.180±0.008)均较对照组减低(P<0.05);用药6个月、12个月两组比较有显著性差异(P<0.05)。结论:MVP可能参与癫痫耐药的发生,LEV可以控制癫痫发作,并可能下调MVP的表达。
Objective ： To investigate the effects of levetiracetam on expression of major vault protein （MVP） in the peripheral blood of children with refractory epilepsy. Methods： From Sep. 2012 to Feb. 2013, 30 patients with refractory epilepsy （ 18 male cases, 12 female cases, age 5.2 ± 1.8 years） were collected in Chirdren＇s Hospital of Wuxi City. The patients had to take levetiracetam over twelve months. They were divided into three groups ： Before taking drug group （ B group）, taking drug for six months group（ C group） and taking drug for twelve months group（ D group）. The 30 healthy children （16 male cases, 14 female cases） to the hospital physical examination were collected as control group （ A group）. The expression of MVP mRNA in their peripheral blood cells was detected by semi-quantitative reverse transcrip- tion- polymerase chain reaction （RT-PCR）, and their protein was detected by Western Blot. The results were processed by statistics. Results： A small amount of MVP expression was detected in the control group （A group, mRNA： 1. 133 ±0. 152, Protein：0. 020 ± 0. 008 ）. MVP protein expression elevated significantly before treatment （B group, mRNA： 1. 788 ± 0. 097, Protein： 0. 209± 0.012）. And there was significant difference as for the MVP expression in between the A group and the B group according to statistics expression trends（P 〈 0.05）. The MVP expression in levetiracetam treatment groups（C group, mRNA： 1. 657 ± 0. 126, Protein： 0. 196 ± 0. 012; D group, mRNA： 1. 466 ± 0. 199, Protein： 0. 180 ±0. 008） was lower than A group（P 〈0.05 ）. There was significant difference as for the MVP expression in between the C group and the D group according to statistics （P 〈 0.05 ）. Conclusion： MVP may play an important role in the development of resistance of refractory epilepsy, LEV can control epileptiform seizure, and reduce the MVP expression.
出处
《神经解剖学杂志》
CAS
CSCD
北大核心
2015年第1期109-113,共5页
Chinese Journal of Neuroanatomy
关键词
主穹窿蛋白
难治性癫痫
左乙拉西坦
外周血
main vault protein refractory epilepsy levetiracetam peripheral blood
