摘要
目的 探讨Toll样受体（TLR）2和4与黄褐斑发病的相关性.方法 黄褐斑患者和健康对照各40例,采集外周血,并收集其中10例黄褐斑及10例健康对照者皮损,用RT-PCR方法检测血样及皮损中TLR2和4mRNA的表达,免疫组化观察TLR2和4在皮损中的表达及分布,并进行统计分析.结果 黄褐斑皮损中TLR2和TLR4在mRNA水平表达（分别为9.72±2.93,9.52±2.88）明显高于健康对照（分别为5.10±2.69,4.77±1.90）,差异有统计学意义（分别t=3.67、4.36,均P＜0.01）.40例黄褐斑患者及40例健康对照外周血中TLR2和TLR4的表达,差异无统计学意义（P＞0.05）.免疫组化显示：10例正常皮肤中6例表皮及血管内皮细胞均无TLR2表达,4例表皮基底层有TLR2弱阳性表达,血管内皮细胞未见表达.10例黄褐斑皮损中有3例表皮全层均有TLR2阳性表达,7例基底细胞层及棘层有TLR2阳性表达,真皮浅层血管内皮细胞未见TLR2表达.10例健康对照皮肤表皮及血管内皮细胞均未见TLR4表达.10例黄褐斑皮损基底细胞层均有TLR4强阳性表达,棘层弱阳性表达,其中3例真皮浅层血管内皮细胞可见阳性TLR-4表达.结论 黄褐斑发病可能与局部皮肤TLR介导的炎症反应有关.
Objective To investigate the relationship of Toll-like receptors （TLRs） 2 and 4 with the occurrence of chloasma.Methods Peripheral blood samples were collected from 40 patients with chloasma and 40 healthy human controls,and skin samples were also collected from the lesions of 10 of the patients and normal skin of 10 of the healthy controls.Real time （RT）-PCR was performed to measure the mRNA expressions of TLR2 and TLR4 in skin lesions and blood samples.An immunohistochemical test was conducted to observe the expressions of TLR2 and TLR4 in skin lesions.Statistical analysis was carried out by t test.Results The expressions of TLR2 and TLR4 mRNAs were both significantly higher in skin lesions of the patients than in normal skin of the controls （9.72 ± 2.93 vs.5.10 ± 2.69,t =3.67,P〈 0.01; 9.52 ± 2.88 vs.4.77 ± 1.90,t =4.36,P〈 0.01）,while no significant difference was found in the mRNA expressions of TLR2 or TLR4 in peripheral blood between the patients and controls （both P 〉 0.05）.As the immunohistochemical test revealed,TLR2 was absent in both the epidermis and vascular endothelial cells in 6 normal control skin samples,weakly expressed in the basal layer of the epidermis but absent in vascular endothelial cells in 4 normal skin samples,and no TLR4 expression was observed in either the epidermis or vascular endothelial cells in these control skin samples.Among the 10 skin samples from chloasma lesions,3 showed TLR2 expression in the whole epidermis,7 in both basal cell layer and prickle cell layer but not in vascular endothelial cells in the superficial dermal layer,all showed strong TLR4 expression in the basal cell layer and weak TLR4 expression in the prickle cell layer,and 3 exhibited TLR4 expression in vascular endothelial cells in the superficial dermal layer.Conclusion TLR-mediated immune responses in local skin might be related to the occurrence of chloasma.
出处
《中华皮肤科杂志》
CAS
CSCD
北大核心
2015年第2期100-103,共4页
Chinese Journal of Dermatology
基金
教育部2013年度创新团队发展计划（IRT13067）
云南省卫生高层次人才培养计划（L-201211）
2012年中华医学会薇姿研究项目（V2012080514）
关键词
黄褐斑
TOLL样受体
炎症
Chloasma
Toll-like receptors
Inflammation
