摘要
目的检测复发性流产患者外周血、绒毛及蜕膜组织中Toll样受体4（TLR4）、CD80的表达状态，探讨其表达与复发性流产的关系，为临床诊断和预防提供理论依据。方法选择2011年3月至2012年1月山西医科大学第一医院妇产科门诊就诊的复发性流产患者60例为研究组，选择同期同孕龄既往有分娩史而无不良妊娠史的自愿行人工流产的正常早孕妇女30例为对照组，分别抽取其外周血，留取绒毛、蜕膜组织，用免疫组化法检测样本中TLR4、CD80的表达情况。结果研究组外周血淋巴细胞、绒毛、蜕膜中TLR4及CD80的表达均明显高于对照组（P〈0．05）；研究组外周血淋巴细胞、绒毛组织、蜕膜组织中TLR4与CD80的表达均呈正相关（r=0．982，0．986，0．776，P〈0．01）。结论复发性流产的发生与外周血淋巴细胞、绒毛及蜕膜组织中TLR4、CD80的高表达密切相关，对早孕妇女进行外周血TLR4、CD80的监测，有利于预测流产发生的风险。
Objective To investigate the expression of Toll-like receptor 4 （TLR4） and CD80 in unexplained recurrent spontaneous abortion and their relationship, discuss its possible roles in guiding eu- genics, and provide a new idea for clinical diagnosis and prevention. Methods The study group were 60 cases with unexplained recurrent spontaneous abortion patients. The control group included 30 cases of normal early pregnancy without previous history of adverse pregnancy. The expressions of TLR4 and CD80 were detected with immunohistochemistry in the peripheral blood, chorion, and decidua from each case. Results TLR4 had higher expression in the peripheral blood, the ehorion and decidua from the recurrent spontaneous abortion women than that from normal pregnancy women （ P 〈 0. 05 ）. Levels of CDS0 were significantly higher in recurrent spontaneous abortion group than in normal pregnancy group （ P 〈 0. 05 ）. There was a positive correlation between TLR4 and CD80 （ r = 0. 982, 0. 986, and 0. 776, P 〈 0.01 ）. Conclusions The study shows that TLR4 and CD80 were expressed in peripheral blood lymphocytes and villi and decidua of early pregnancy （normal pregnancy or abnormal pregnancy）. Higher expressions of TLR4 and CD80 might play an important role in the occurrence of recurrent spontaneous abortion.
出处
《中国医师杂志》
CAS
2015年第1期68-70,共3页
Journal of Chinese Physician
