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基于生物信息学方法挖掘奶山羊miRNAs研究 被引量：4

In silico detection and characteristics of miRNAs in dairy goat
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摘要 microRNAs(miRNAs)是长约22nt的内源非编码小分子RNA,在转录后基因调控中发挥重要作用。奶山羊是具有重要经济价值的产乳动物,有关奶山羊miRNAs研究相对匮乏,识别和鉴定新的奶山羊miRNA至关重要。文章以与奶山羊高度同源的绵羊基因组为参考数据库,应用生物信息学方法得到101条新的奶山羊miRNAs序列,并对其进行序列特性分析,为今后基因组信息不全物种的miRNAs挖掘与鉴定提供参考。 micro RNAs（mi RNAs） are a large class of endogenous non- coding small RNAs that average 22 nucleotides（nt） in length, which play important roles in post- transcriptional gene regulation because they can negatively regulate gene expression. Dairy goat is a milk producing animal with economic importance. The studies on mi RNAs in dairy goat are relatively lack and then detecting and identifying the new mi RNAs of dairy goat is very important. In this study, 101 mi RNAs of dairy goat were obtained using bioinformatics approach based on sheep genome, which was highly homologous with goat. Finally, detailed analysis of sequence characteristics in novel mi RNAs of goat were carried out. This study would provide a reference for further identification of mi RNAs in animals without complete genome.
作者 曲波 甄贞 仇有文 袁肖寒 王春梅
机构地区 东北农业大学生命科学学院
出处 《东北农业大学学报》 CAS CSCD 北大核心 2015年第1期86-93,共8页 Journal of Northeast Agricultural University
基金 国家自然科学基金项目(31100959) 黑龙江省博士后启动基金项目(LBH-Q11169)
关键词 MIRNAS 奶山羊 绵羊基因组 生物信息学方法 mi RNAs dairy goat sheep genome bioinformatics approach
分类号 Q492.7 [生物学—生理学] S858.2 [农业科学—临床兽医学]
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参考文献12

  • 1Frazier T P,Zhang B.Identification of plant micro RNAs using expressed sequence tag analysis[J].Methods Mol Biol,2011,678:13-25.
  • 2Zhou B,Liu H L.Computational identification of new porcine micro RNAs and their targets[J].Anim Sci J,2010,81(3):290-296.
  • 3Lewis B P,Burge C B,Bartel D P.Conserved seed pairing,often flanked by adenosines,indicates that thousands of human genes are micro RNA targets[J].Cell,2005,120:15-20.
  • 4Friedman R C,Farh K K,Burge C B,et al.Most mammalian m RNAs are conserved targets of micro RNAs[J].Genome Res,2009,19(1):92-105.
  • 5金晓露,杨建香,李真,刘红云,刘建新.乳腺发育及泌乳相关miRNA研究进展[J].遗传,2013,35(6):695-702. 被引量：20
  • 6Barozai M Y.The novel 172 sheep(Ovis aries)micro RNAs and their targets[J].Mol Biol Rep,2012,39(5):6259-6266.
  • 7Mendes N D,Freitas A T,Sagot M F.Current tools for the identification of mi RNA genes and their targets[J].Nucleic Acids Res,2009,37(8):2419-2433.
  • 8Bartel D P.Micro RNAs:Genomics,biogenesis,mechanism,and function[J].Cell,2004,116(2):281-297.
  • 9Kaftanovskaya H M,Serov O L.High-resolution GTG-banded chromosomes of cattle,sheep,and goat:A comparative study[J].J Hered,1994,85(5):395-400.
  • 10Kozomara A,Griffiths-Jones S.mi RBase:Integrating micro RNA annotation and deep-sequencing data[J].Nucleic Acids Res,2011,39:152-157.

二级参考文献46

  • 1Lee RC, Feinbaum RL, Ambros V. The C. elegans hetero- chronic gene lin-4 encodes small RNAs with antisense complementarity to lin-14. Cell, 1993, 75(5): 843-854.
  • 2Wightman B, Ha I, Ruvkun G. Posttranscriptional regulation of the heterochronic gene lin-14 by lin-4 mediates temporal pattern formation in C. elegans. Cell, 1993, 75(5): 855-862.
  • 3Reinhart B J, Slack F J, Basson M, Pasquinelli AE, Bettinger JC, Rougvie AE, Horvitz HR, Ruvkun G. The 21-nucleotide let-7 RNA regulates developmental timing in Caenorhabditis elegans. Nature, 2000, 403(6772): 901- 906.
  • 4Hennighausen L, Robinson GW. Information networks in the mammary gland. Nat Rev Mol Cell Biol, 2005, 6(9): 715 725.
  • 5Bartel DP. MicroRNAs: genomics, biogenesis, mechanism, and function. Cell. 2004. 116(2": 281-297.
  • 6Liu CG, Calin GA, Meloon B, Gamliel N, Sevignani C, Ferracin M, Dumitru CD, Shimizu M, Zupo S, Dono M, Alder H, Bullrich F, Negrini M, Croce CM. An oligonucleotide microchip for genome-wide microRNA profiling in human and mouse tissues. Proc Natl Acad Sci USA, 2004, 101(26): 9740-9744.
  • 7Silveri L, Tilly G, Vilotte JL, Le Provost F. MicroRNA involvement in mammary gland development and breastcancer. Reprod Nutr Dev, 2006, 46(5): 549-556.
  • 8Gu ZL, Eleswarapu S, Jiang HL. Identification and characterization of microRNAs from the bovine adipose tissue and mammary gland. FEBSLett, 2007, 581(5): 981- 988.
  • 9Sdassi N, Silveri L, Laubier J, Tilly G, Costa J, Layani S, Vilotte JL, Le Provost F. Identification and characteriza- tion of new miRNAs cloned from normal mouse mammary gland. BMC Genomics, 2009, 10: 149.
  • 10Li Z, Liu HY, Jin XL, Lo LJ, Liu JX. Expression profiles of microRNAs from lactating and non-lactating bovine mammary glands and identification of miRNA related to lactation. BMC Genomics, 2012, 13:731.

共引文献19

同被引文献65

  • 1陈芳,殷勤伟.调控基因表达的miRNA[J].科学通报,2005,50(13):1289-1299. 被引量：21
  • 2Aili Li,Long Mao.Evolution of plant microRNA gene families[J].Cell Research,2007,17(3):212-218. 被引量：14
  • 3Schlueter J A,Dixon P,Granger C,et al.Mining EST databases to resolve evolutionary events in major crop species[J].Genome,2004,47(5):868-876.
  • 4Wang L,Cao C,Ma Q,et al.RNA-seq analyses of multiple meristems of soybean:Novel and alternative transcripts,evolutionary and functional implications[J].BMC plant biology,2014,14(6):150-169.
  • 5Bartel D P.Micro RNAs:Genomics,biogenesis,mechanism,and function[J].Cell,2004,116(2):281-297.
  • 6Wang J W,Czech B,Weigel D.mi R156-regulated SPL transcription factors define an endogenous flowering pathway in Arabidopsis thaliana[J].Cell,2009,138(4):738-749.
  • 7Zhang B,Pan X,Cobb G P,et al.Plant micro RNA:A small regulatory molecule with big impact[J].Developmental Biology,2006,289(1):3-16.
  • 8Sunkar R,Li Y F,Jagadeeswaran G.Functions of micro RNAs in plant stress responses[J].Trends in Plant Science,2012,17(4):196-203.
  • 9Schmutz J,Cannon S B,Schlueter J,et al.Genome sequence of the palaeopolyploid soybean[J].Nature,2010,463(7278):178-183.
  • 10Zhao M,Meyers B C,Cai C,et al.Evolutionary patterns and coevolutionary consequences of MIRNA genes and micro RNA targets triggered by multiple mechanisms of genomic duplications in soybean[J].The Plant Cell,2015,27(3):546-562.

引证文献4

二级引证文献8

东北农业大学学报
2015年 第1期

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