摘要
目的研究真菌Aspergillus sp.YN-3次级代谢产物的化学成分。方法采用硅胶柱色谱法、制备薄层色谱法、Sephadex LH-20凝胶柱色谱法和HPLC法等方法进行对真菌Aspergillus sp.YN-3发酵产物的分离纯化,根据理化性质、NMR、MS等波谱数据鉴定化合物的结构,并采用台盼蓝法对化合物1～3进行了人早幼粒白血病细胞株HL-60的生长抑制实验。结果分离得到10个化合物,分别鉴定为：chrodrimanin E（1）、thailandolide B（2）、thailandolide A（3）、3-（3-羟基-5-甲基苯氧基）-5-甲基苯酚[3-（3-hydroxy-5-methylphenoxy）-5-methylphenol,4]、2,3-二羟基苯甲酸（2,3-dihydroxybenzoic acid,5）、3-羟基-2-甲基苯甲酸（3-hydroxy-2-methylbenzoic acid,6）、环（色-苯丙）二肽[cyclo（Trp-Phe）,7]、环（色-亮）二肽[cyclo（Trp-Leu）,8]、（22E,24R）-麦角甾-5,7,22-三烯-3β-醇[（22E,24R）-ergosta-5,7,22-trien-3β-ol,9]和3-乙酰氨基-5-乙酰呋喃（3-acetamido-5-acetylfuran,10）。结论化合物1～3从该属真菌中首次分离得到,化合物10为一新天然产物;化合物1～3对于HL-60细胞株显示了较弱的生长抑制活性。
Objective To study the secondary metabolites of fungus Aspergillus sp.YN-3.Methods Compounds were isolated by silica gel column chromatography,Sephadex LH-20 column chromatography,preparative TLC and HPLC.Their structures were identified by analysis of the physico-chemical characters and spectral data.The growth inhibitory effects of compounds 1-3 against HL-60 cell line were tested by trypanblue staining assay.Results Ten compounds were obtained and identified as chrodrimanin E（1）,thailandolide B（2）,thailandolide A（3）,3-（3-hydroxy-5-methylphenoxy）-5-methylphenol（4）,2,3-dihydroxybenzoic acid（5）,3-hydroxy-2-methylbenzoic acid（6）,cyclo-（Trp-Phe）（7）,cyclo（Trp-Leu）（8）,（22 E,24R）-ergosta-5,7,22- trien-3β-ol（9）,3-acetamido-5-acetylfuran（10）,respectively.Conclusions Compounds 1-3 are isolated from Aspergillus genus for the first time,and compound 10 is obtained as a natural product for the first time.Compounds 1-3 show w eak growth inhibitory activities against HL-60 cell line.
出处
《沈阳药科大学学报》
CAS
CSCD
北大核心
2015年第2期111-115,共5页
Journal of Shenyang Pharmaceutical University
关键词
真菌
曲霉属
次级代谢产物
抗肿瘤活性
fungus
Aspergillus sp.
secondary metabolites
antitumor activity
