摘要
In this study,with Saccharomyces cerevisiae as the experimental material,microwave-assisted technology,phenol-sulfuric acid method and UV spectrophotometry were employed to investigate the extraction rate of polysaccharides. Solid-liquid ratio,microwave power and microwave processing time were adopted as single factors to optimize the extraction conditions. Subsequently,orthogonal experiment was performed to investigate the optimal extraction process of polysaccharides from S. cerevisiae. The optimal extraction conditions were: solid-liquid ratio 1∶ 20,microwave power 250 W,microwave processing time 3 min,under which the extraction rate of polysaccharides reached 4. 98%. Compared with conventional hot water extraction method,microwave-assisted extraction technology exhibited shorter extraction time,higher efficiency,and could save more energy.
In this study, with Saccharomyces cerevisiae as the experimental material, microwave-assisted technology, phenol-sulfuric acid method and UV spectro- photometry were employed to investigate the extraction rate of polysaccharides. Solid-liquid ratio, microwave power and microwave processing time were adopted as single factors to optimize the extraction conditions. Subsequently, orthogonal experiment was performed to investigate the optimal extraction process of polysaccha- rides from S. cerevisiae. The optimal extraction conditions were： solid-liquid ratio 1 ： 20, microwave power 250 W, microwave processing time 3 min, under which the extraction rate of polysaccharides reached 4.98%. Compared with conventional hot water extraction method, microwave-assisted extraction technology exhibited shorter extraction time, higher efficiency, and could save more energy.
基金
Supported by Science and Technology Project of Shandong Province(2011GS2114)
Science and Technology Project of Heze City(2010S002)
