摘要
miRNA是一种内源性小分子非编码RNA,含19-23个核苷酸,通过与mRNA的3＇非编码区结合从而阻断翻译,调节植物或动物的基因表达,并在细胞发育过程中包括细胞裂解、增殖、凋亡及诱导疾病发挥重要的作用.因此,miRNA迅速成为生命科学研究的新领域.而检测miRNA的表达是研究miRNA功能的第一步.表面等离子体共振成像（surface plasmon resonance imaging,SPRI）技术是一种基于光学原理的传感检测技术,它利用表面等离子体共振原理,可将生物分子相互作用或生化反应的信号转化成光信号进行传感,具有无需标记、实时检测、快速、高通量检测等优点,在miRNA的检测上将显示出巨大的发展潜力和应用价值.本文对目前miRNA的检测方法,以及高通量SPRI技术在miRNA检测中的应用发展趋势做一综述.
In plants and animals,micro-RNAs（ miRNA） are a group of small,non-coding RNA（ 19 -23 nucleotides） that bind to the 3＇-untranslated region of targeted mRNA to regulate genes expression.Various miRNAs play a critical role in the processes of cell differentiation,proliferation and apoptosis,or in diseases. To understand the role of miRNAs,the expression level of miRNAs was detected via surface plasmon resonance imaging（ SPRI）,which is a technology based on the generated optical signals from bio-molecular interaction or bio-chemical reaction. The advantages of SPRI are label-free,real-time,fast and high-throughput when being applied for miRNA detections,especially. This review discussed the recent advances in the areas of miRNA detection in the first part. The development and application of high-throughput SPRI technique for miRNA detection was introduced in the second part.
出处
《中国生物化学与分子生物学报》
CAS
CSCD
北大核心
2015年第2期146-152,共7页
Chinese Journal of Biochemistry and Molecular Biology
基金
国家质检总局科技计划项目(No.2014IK042
201310087
2013IK237)
广东省科技计划项目(No.2012B031800126,2013-cg-31)
深圳市科技计划项目(No.ZYA201106080020A,No.JCYJ20120618172144495,SGLH20121008 144756945,CXZZ20130322112111131)~~
