摘要
目的观察n-3多不饱和脂肪酸(n-3PUFAs)对大鼠大肠癌及Caspase-3蛋白表达的影响。方法将60只SD大鼠随机分成对照组和实验组,实验组给予n-3PUFAs灌胃,对照组给予等量的生理盐水灌胃,两组均使用N-甲基-N-亚硝基脲(MNU)进行灌肠。16周后解剖大鼠,观察肿瘤情况,HE切片明确肿瘤的病理,气相色谱测定红细胞n-3PUFAs的浓度,免疫组化检测癌组织及正常组织Caspase-3蛋白的表达。结果实验组大鼠的红细胞n-3PUFAs浓度高于对照组(P<0.01),肿瘤发生率低于对照组(P<0.05),Caspase-3蛋白表达高于对照组(P<0.05),差异均有统计学意义。结论 n-3PUFAs能够减少MNU诱导大鼠大肠癌的发生率,可能机制是增加癌细胞凋亡。
Objective To investigate the effects of n-3 polyunsaturated fatty acids（n-3PUFAs） on colorectal cancer in SD rats and Caspase- 3 protein expression. Methods Sixty SD rats were randomly divided into control group and experimental group,in which saline and n- 3PUFAs were given as treatment,respectively. MNU enema was given in both groups. The pathological observation was performed at the 16 thweekby HE sections. The n- 3PUFAs concentration on erythrocyte membrane was measured by gas chromatography. Caspase- 3 protein expression was measured by immunohistochemical detection. Results Significant elevation of n- 3PUFAs concentration was observed in experimental group,with significant reduction in colorectal cancer prevalence and up- regulation in Caspase- 3. Conclusion Via induction of tumor apoptosis,n- 3PUFAs prevents the occurrence of colorectal cancer in SD rats.
出处
《广东医学》
CAS
CSCD
北大核心
2014年第13期1987-1989,共3页
Guangdong Medical Journal
基金
广东省湛江市2011年度财政资金科技专项竞争性分配项目(编号:湛科[2011]79号-58)
