摘要
棉花是一种重要的经济作物,在国民生产中起着重要的作用。然而干旱盐碱等不良环境严重地制约着棉花的种植区域及其产量和品质。近年来,利用分子育种改良棉花对这些逆境的耐性的研究成为热点。本文从棉花中克隆了一个对干旱和盐碱具有应答的WRKY基因,定名为GhWRKY25。该基因编码的蛋白包含两个典型的WRKY结构域,每个WRKY结构域的C端含有一个C2H2类型的锌指蛋白结构,进化树分析表明其属于Ⅰ类WRKY蛋白,与可可的WRKY3同源性最高。利用qPCR分析发现该基因在棉花里为组成型表达,但在花中表达量较高。GhWRKY25在施用SA、JA、ABA、GA中表达表现为上调。干旱和NaCl处理时发现GhWRKY25基因表达均显著上调。通过上述结果可以推测GhWRKY25在棉花对于逆境的适应中具有重要的作用,为该基因功能的进一步研究提供基础。
Cotton（Gossypium hirsutum L.） is an important economic crop,which plays pivotal roles in industry and social life.However,many uncontrolled environmental factor,such as drought and saline,affect plant area,yield and quality of cotton.Currently,molecular breeding for improving the tolerance of cotton cultivars against various stresses has become the focus of research. In this study,a novel WRKY gene was cloned from cotton,designated as GhWRKY25. The GhWRKY25 protein contains two typical WRKY domain,in which a zinc finger motif is located in the C-terminal. Phytogenic analysis showed that GhWRKY25 belongs to the class Ⅰ WRKY protein,and has highest homology with TcWRKY3 in Theobroma cacao. The results of qPCR analysis showed that GhWRKY25 is constitutively expressed in various cotton organs with high mRNA level in flowers.The expression of GhWRKY25 was up-regulated in cotton plants under the external-applying SA,JA,ABA and GA3. When cotton plants were treated with drought and NaCl,the expression level of GhWRKY25 significantly increased compared with the control.The above results showed that GhWRKY25 is able to response to various hormones,drought and salt stresses,indicating that it could play important roles in cotton tolerance to different stress,and provide a basis for futher reaearch on GhWRKY25 function.
出处
《生物技术进展》
2014年第4期274-279,共6页
Current Biotechnology
基金
山西省自然基金项目(2012011030-1)
山西省青年科技研究基金项目(2012021023-2)资助
关键词
棉花
转录因子
GhWRKY25
抗逆
植物激素
upland cotton
transcription factor
GhWRKY25
stress tolerance
phytohormone
