摘要
通过常规组织分离法从带病枣果中分离得到32株细菌菌株,采用平板对峙法筛选出对枣果黑斑病(Alternaria alternata)病原菌抑菌率达86%的拮抗细菌菌株xj063-1。通过对菌株的形态学观察,生理生化测定结合分子生物学方法,将拮抗菌株xj063-1鉴定为枯草芽孢杆菌(Bacillus subtilis)。通过致畸菌丝的形态观察,发现抑制机理主要为抑制病原菌孢子产生和萌发,使得菌丝畸形。
Thirty two bacteria strains were obtained from diseased jujube fruits by normal tissue separa-tion method,the flat confrontation method were applied to screen antagonistic bacteria strain xj063-1 whose bacteriostatic rate was 86% for Alternaria alternata .Observation of the morphology of the bacteria strains and experiment of physiology and biochemistry were conducted to identify antagonistic bacteria strain xj063-1 as bacillus sbtilis by molecule biological characteristic method.Observation of teratogenic hyphae were applied to find the inhibition mechanism mainly were production and germination of inbihition spore to make hyphae deformed.
出处
《新疆农业大学学报》
CAS
2014年第6期460-464,共5页
Journal of Xinjiang Agricultural University
基金
新疆农业大学大学生创新项目(jqzyp62012134)
森林培育重点学科项目
新疆维吾尔自治区林果实验教学示范中心建设项目
关键词
枣果黑斑病菌
拮抗细菌
筛选
鉴定
Alternaria alternate
antagonistic
bacterial
screening
identification
