摘要
目的通过模拟阻塞性睡眠呼吸暂停低通气综合征的发病特征,建立大鼠间歇性低氧(IH)模型,观察慢性IH早期大鼠海马CA1区低氧诱导因子1(HIF-1)、存活素(survivin)的表达及细胞凋亡情况。方法72只雄性Wistar大鼠随机均分为对照组和间歇低氧(50mL/LIH)组,对照组向低氧箱内持续注入压缩空气,间歇低氧组分别暴露于间歇性低氧条件下(暴露时间每天7h,持续时间分别为3、7、14、21d)。免疫组织化学染色检测海马CA1区HIF-1、survivin蛋白的表达,原位末端标记法检测神经细胞凋亡。结果与对照组比较,海马CA1区神经细胞凋亡指数在间歇低氧3d时无明显差异,间歇低氧7、14、21d组凋亡指数均明显高于对照组,于21d达高峰。与对照组比较,间歇低氧组HIF-1、survivin蛋白的表达在各时间点均增加,14d达峰值后逐渐下降,两者表达呈正相关关系(r=0.836)。结论早期间歇低氧可诱导海马CA1区HIF-1、survivin蛋白的表达,二者在神经细胞凋亡的调控中可能有一定作用。
Objective To establish an intermittent hypoxia （IH） animal model by simulating the features of obstructive sleep apnea-hypopnea syndrome and observe the expressions of hypoxia-inducible factor-1 （ HIF-1 ） and survivin and the occurrence of apoptosis in the hippocampal CA1 neuron of rats with early IH. Methods Male Wistar rats （n = 72） were randomly divided into control group and IH group. In control group, rats were given compressed air and in IH group, rats were exposed to 50 mL/L IH for 7 hours a day, lasting 3, 7, 14 and 21 days, respectively. The expressions of HIF-1 and survivin in hippocampal CA1 were detected by immunohistochemistry and the neuronal apoptosis index was calculated by TUNEL. Results Compared with control group, apoptosis index in the third day of 5% IH group had no significant difference, while apoptosis index in IH 7-, 14- and 21-day groups were significantly higher than that in control group, and on the 21st day it reached a peak. The expressions of HIF-1 and survivin protein in 5% IH group significantly increased as compared with control group at each time point, and they peaked on the 14th day, then decreased gradually. There was a positive relationship between the expressions of HIF-1 and survivin. Conclusion Early IH can induce HIF-1 and survivin protein expressions in rat hippocampal CA1, and their expressions may play an important role in the regulation of neuronal apoptosis.
出处
《细胞与分子免疫学杂志》
CAS
CSCD
北大核心
2015年第3期338-341,共4页
Chinese Journal of Cellular and Molecular Immunology
基金
河北省自然科学基金(H2014209231)
河北省重大医学科研课题(zd2013091)
