摘要
目的 探讨长期连续天然放射性照射对人群氧化损伤及抗氧化水平的影响.方法 选择广东天然放射性高本底辐射地区（HBRA）48名男性居民为研究对象,选择恩平市某镇（CA）相匹配的48名男性居民为对照人群.采集2组人群外周静脉血并分离血浆,采用酶联免疫吸附试验（ELISA）,测定血浆中DNA氧化损伤指标8-羟基脱氧鸟苷（8-OHdG）和抗氧化指标硫氧还蛋白还原酶（TrxR）的表达水平.结果 与对照组相比,高本底地区人群外周血血浆中DNA氧化损伤指标8-OHdG表达水平由（315.39±100.59） ng/ml降低至（272.64±96.85） ng/ml,抗氧化指标TrxR表达水平由（0.467±0.056） ng/ml升高至（0.496±0.044） ng/ml,两组间的差异均有统计学意义（t=2.121、-2.823,P＜0.05）.多元线性回归分析结果显示,在排除年龄、饮酒、喝茶、吸烟、接受医疗照射、生活应激事件等混杂因素的影响后,低剂量电离辐射个人累积剂量对8-OHdG和TrxR表达水平均有影响（t=-2.327、2.367,P＜0.05）.结论 长期接触低剂量电离辐射可降低人群氧化损伤水平,增强机体抗氧化水平.
Objective To investigate the effects of low dose radiation on the level of oxidative damage and antioxidant in population of high background radiation area of Guangdong.Methods A total of 48 male residents who lived in high background radiation area（HBRA） of Guangdong province and 48 male residents who lived in neighboring Enping control area were chosen as the objectives and control respectively.The peripheral venous blood of two groups was collected,and then the levels of 8-OHdG and TrxR were determined by enzyme linked immunosorbent assay （ELISA）.Results Compared with the CA group [（315.39 ± 100.59） ng/ml],the level of 8-OHdG [（272.64 ± 96.85） ng/ml] decreased significantly in HBRA （t =2.121,P 〈0.05）.Compared with the CA group [（0.467 ±0.056） ng/ml],the level of TrxR [（0.496 ± 0.044） ng/ml] increased significantly in HBRA （t =-2.823,P 〈 0.05）.The results of multiple linear regression analysis demonstrated that the chronic exposure to low dose of radiation had significant effects on the expression level of 8-OHdG and TrxR （t =-2.327,2.367,P 〈 0.05） after adjustment for confounding factors such as age,drinking,tea drinking,smoking,medical exposure and stressful events.Conclusions Chronic exposure to low dose radiation may decrease the level of oxidative and enhance the level of antioxidant.
出处
《中华放射医学与防护杂志》
CAS
CSCD
北大核心
2015年第2期83-87,共5页
Chinese Journal of Radiological Medicine and Protection
基金
国家自然科学基金（81302387）
广东省医学科研基金（B2013022）
苏州大学高校省级重点实验室开放课题（KJS1131）
