摘要
目的观察从肺论治法和从肠论治法对溃疡性结肠炎(ulcerative colitis,UC)大鼠肺与结肠中血管活性肠肽(vasoactive intestinal peptide,VIP)含量的影响。方法选取52只大鼠采用家兔结肠黏膜组织致敏与三硝基苯磺酸(TNBS)-乙醇模型相结合的免疫复合法制备UC大鼠模型(造模成功率为78%)。从造模成功的40只大鼠及16只正常组大鼠中随机各选出8只作为干预前(0周时间点)模型组及正常组。剩余造模成功的32只大鼠随机分为模型组、西药组(柳氮磺胺吡啶)、从肺论治组(黄芪桔梗汤)、从肠论治组(黄芪黄连汤),每组8只,各治疗组均按60 kg体重成人药量8倍剂量灌胃给药;另取8只正常大鼠为正常组,模型组、正常组以等体积纯净水灌胃,每天1次;两组均连续干预4周(4周时间点)后,分别于0周、4周时间点采用放射免疫法测定肺与结肠组织中VIP的含量。并在光学显微镜下观察肺组织及结肠组织病理形态学改变。结果与正常组同期比较,模型组0周时间点肺间质可见均匀弥漫性炎症,支气管壁增厚,支气管、血管周围大量淋巴细胞浸润,肠黏膜大面积坏死及炎性细胞浸润,干预后各治疗组肺及结肠组织病理损伤较模型组减轻;与正常组同期比较,模型组4周末肺组织VIP含量降低(P<0.05),模型组0周、4周末结肠组织VIP含量升高(P<0.05)。与模型组比较,西药组、从肺论治组4周末肺组织VIP含量升高(P<0.01),从肺论治组、从肠论治组结肠组织VIP含量降低(P<0.05,P<0.01)。结论从肺论治法和从肠论治法通过调节肺及结肠组织中VIP含量,在改善肺及肠道局部炎症,减轻病理损伤、促损伤修复方面优势突出。
Objective To observe effects of treatment from the lung and treatment from the intestine on the level of vasoactive intestinal peptide （VIP） in the lung and intestine of ulcerative colitis （UC） rats. Methods The UC rat model was established in 52 rats by using rabbit intestine mucosa tissue allergen combined TNBS-ethanol model （with the model successful rate of 78. 0%）. Eight rats randomly selected from 40 successfully modeled rats and 8 of 16 rats from the normal group were recruited as the model group and the normal control group before intervention （at week 0）. The rest 32 successfully modeled rats were randomly divid- ed into the model group, the Western medicine treatment group （salazosulfapyridine）, the treatment from lung group （Huangqi J iegeng Decoction）, and the treatment from intestine group （Huangqi Huanglian Decoction）,8 in each group. Rats in each treatment group were administered with corresponding medication 8 times the dose of a 60 kg adult human. Another 8 normal rats were recruited as the normal group. Equal volume of pure waterwas given to rats in the model group and the normal group by gastrog avage, once per day. Contents of VIP in the lung tissue and the intestinal tissue were detected at week 0 and 4 after 4-week consecutive intervention. Pathomorphological changes of the lung tissue and the colon tissue were observed under light microscope. Re- sults Compared with the normal control group at week 0, evenly distributed diffuse inflammation could be seen in the pulmonary interstitial tissue; the bronchial wall was thickened; a huge amount of infiltration surrounded bronchi and blood vessels ; a large area of necrosis of intestinal mucosa and inflammatory cell infiltration could also be seen in the model group. Pathological injuries of the lung and the colon were more alleviated in each treatment group than in the model group at the same time point. Compared with the normal control group at the same time point, VIP contents in the lung tissue significantly decreased in the model group at the end of week 4 （P 〈0.05） ; VIP contents in the colon tissue significantly increased in the model group at the end of week 0 and 4 （P 〈0. 05）. Compared with the model group, VIP contents in the lung tissue significantly in creased in the Western medicine treatment group and the treatment from lung group at the end of week 4 （P 〈 0. 01 ） ; VIP contents in the colon tissue significantly decreased in the treatment from lung group and the treatment from intestine group （P 〈0.05, P 〈0. 01 ）. Conclusion Treatment from the lung and treatment from the intestine showed predominant advantage in improving local inflammation of the lung and the intestinal tract, alleviating pathological injuries, promoting repair of injuries through regulating VIP contents in the lung tissue and the colon tissue.
出处
《中国中西医结合杂志》
CAS
CSCD
北大核心
2015年第2期222-227,共6页
Chinese Journal of Integrated Traditional and Western Medicine
基金
国家重点基础研究发展计划(973计划)资助项目(No.2009CB522705)
河南中医学院博士科研基金资助项目(No.BSJJ2011-19)
关键词
溃疡性结肠炎
从肺论治法
从肠论治法
血管活性肠肽
肺损伤
ulcerative colitis
treatment from the lung
treatment from the intestine
vasoactive in-testinal peptide
lung injury
