摘要
目的:研究GRP-78和caspase-12在氟致大鼠成釉细胞内质网应激和细胞凋亡中的作用,探讨氟所介导的内质网应激是否导致细胞凋亡。方法:应用CCK8和流式细胞术观察不同浓度的氟对成釉细胞活性及凋亡数量的影响,以实时定量PCR和Western印迹法检测氟对内质网伴侣分子GRP-78和caspase-12基因和蛋白表达的影响,应用SPSS13.0软件包对数据进行统计学分析。结果:随着氟浓度的不断增加,大鼠成釉细胞活性呈逐渐下降趋势,流式细胞术同样显示发生凋亡的细胞数量逐渐上升;实时定量RT-PCR和Western印迹结果显示,GRP-78和caspase-12的表达量随着氟浓度增加而增加。结论:过量氟介导了大鼠成釉细胞内质网应激,并且通过内质网应激,引起细胞凋亡。
PURPOSE： To study the effect of GRP-78 and caspase-12 on fluoride-induced endoplasmic reticulum stress and apoptosis in rat ameloblast, and explore whether fluoride-induced endoplasmic reticulum stress results in the occurrence of apoptosis. METHODS： The cell activity of ameloblast cultured in various concentrations of fluoride was measured by CCK8 and flow cytometry; Real-time RT-PCR and Western blot were used to analyze the endoplasmic reticulum chaperone GRP-78 and caspase-12 genes and the expression of related proteins. The data was analyzed with SPSS13.0 software package. RESULTS： With the increasing concentration of fluoride, the cell activity of rat ameloblasts decreased gradually,and flow cytometry also showed that the number of apoptosis gradually increased; Real-time RT-PCR and Western blot showed the expression of GRP-78 and caspase-12 increased while the fluoride concentration increased. CONCLUSIONS：Excessive fluoride induces endoplasmic reticulum stress of rat ameloblast, and leads to cell apoptosis.
出处
《上海口腔医学》
CAS
CSCD
2015年第1期1-5,共5页
Shanghai Journal of Stomatology
基金
国家自然科学基金(81072245)
辽宁省自然科学基金(20102278)~~
关键词
氟
成釉细胞
内质网应激
细胞凋亡
Fluoride
Ameloblast
Endoplasmic reticulum stress
Apoptosis
