摘要
背景:小肠移植过程中移植肠不可避免地要受到缺血再灌注的损害,而小肠又对缺血再灌注损伤特别敏感。前期研究证实丙酮酸对小肠和移植小肠缺血再灌注损伤具有保护作用,并探讨了部分相关机制。目的:在前期研究基础上,进一步证实探讨丙酮酸对大鼠移植小肠缺血再灌注损伤的保护作用与移植小肠组织中细胞间黏附分子表达的关系。方法:选用近交系成年雄性SD大鼠,按随机数字表法分为3组:假手术组行剖腹、左侧肾切除作为对照;移植小肠缺血再灌注组和丙酮酸处理组均建立小肠移植缺血再灌注动物模型,分别于供体小肠阻断血流、灌洗前10 min向肠腔内注入10 m L含有多聚葡萄糖的营养液或含有分析纯丙酮酸的营养液。分别留取缺血45,90 min和再灌注30,180 min小肠组织标本,光镜下观察小肠组织损伤病理变化,免疫组化法检测小肠组织中细胞间黏附分子1的表达。结果与结论:缺血再灌注不同时相移植小肠缺血再灌注组小肠组织损伤程度均重于其他2组,而丙酮酸处理组小肠组织损伤程度与假手术组相似。缺血期间小肠组织中细胞间黏附分子1的表达均不明显,呈弱阳性;再灌注后移植小肠缺血再灌注组细胞间黏附分子1的表达迅速增加,高于与假手术组及丙酮酸处理组(P<0.01),而丙酮酸处理组细胞间黏附分子1的表达变化不明显(P>0.05)。说明丙酮酸作用下大鼠移植小肠缺血再灌注过程中细胞间黏附分子1的表达减少,可能是丙酮酸保护大鼠移植小肠缺血再灌注损伤的重要环节之一。
BACKGROUND：Grafted smal bowels are sensitive to ischemia-reperfusion injury, which is inevitable during transplantation process. Our previous study found the protective effect of pyruvate to rat grafted smal bowel and investigated the underlying mechanisms.
OBJECTIVE：To study the relationship between pyruvate protective effect on ischemia-reperfusion injury and expression of intercel ular adhesion molecular 1 of grafted smal bowel.
METHODS：Adult male Sprague-Dawley rats of inbred line were randomly divided into three groups. Sham operation group：Rats underwent the laparotomy and the left nephrectomy. Ischemia/reperfusion group and pyruvate group：Ischemia/reperfusion rat models of smal bowel transplantation were established and received lavage injection of nutrient solution only or nutrient solution with pyruvate, before donor intestinal blocking blood flow. Smal intestinal tissue was harvested at 45, 90 minutes after ischemia and 30, 180 minutes after reperfusion. The pathological changes of smal intestinal injury were observed under light microscope. The expression of intercel ular adhesion molecular 1 was detected by immunohistochemistry in the smal intestine tissue samples.＆amp;nbsp;RESULTS AND CONCLUSION：The pathological injury of smal intestine tissue at different time points of ischemia/reperfusion was severer in the ischemia/reperfusion group than that in sham operation group and pyruvate group, and there was no significant difference in the latter two groups. The expression of intercel ular adhesion molecular 1 stayed at a low level during ischemia period, and appeared sharp rise during reperfusion period, which was higher than that in the sham operation group and pyruvate group （P〈0.01）. No significant differences were found between sham operation group and pyruvate group in the expression （P〉0.05）. We conclude that pyruvate can afford protective effect to ischemia/reperfusion injury of grafted smal bowel. The mechanism of protective effect of pyruvate is associated with decrease expression of intercel ular adhesion molecular 1.
出处
《中国组织工程研究》
CAS
北大核心
2015年第5期783-787,共5页
Chinese Journal of Tissue Engineering Research
