摘要
目的研究基质金属蛋白酶2（MMP-2）基因启动子区1306C/T和735C/T多态性与动脉粥样硬化性脑梗死初发及复发的相关性。方法选取初发脑梗死350例,复发脑梗死340例,正常健康组360例。采用ELISA法检测MMP-2的含量,聚合酶链反应-限制性片段长度多态性（PCR-RFLP）检测MMP-2基因1306C/T和735C/T的基因型,比较3组间MMP-2基因多态性频率分布及含量的差异。结果初发脑梗死组及复发脑梗死组血清MMP-2水平显著高于对照组[（2.93±0.53）vs（1.68±0.24）mg/L,P〈0.01;（3.32±0.94）vs（1.68±0.24）mg/L,P〈0.01],且复发脑梗死组高于初发脑梗死组[（3.32±0.94）vs（2.93±0.53）mg/L,P〈0.01]。MMP-2 735C/T中复发脑梗死组C等位基因频率明显升高,与初发脑梗死组相比（92.5%vs 87.9%,χ2=4.398,P〉0.01）差异无统计学意义,但与对照组相比（92.5%vs 78.5%,χ2=8.121;P〈0.01）差异有统计学意义;初发脑梗死组与对照组相比（87.9%vs 78.5%,χ2=4.121;P〉0.01）差异无统计学意义。3组MMP-2 1306C/T基因型（χ2=1.075,P〉0.01）和等位基因（χ2=1.124,P〉0.01）的频率分布无统计学意义。多变量Logistic回归分析显示,MMP-2 735C/T多态性是脑梗死复发的独立危险因素（OR：1.765,95%CI：1.177~2.676,P=0.006）。结论MMP-2基因735C/T的C等位基因是脑梗死遗传易感基因之一,可能是脑梗死复发的潜在危险因素。
Objective To investigate the correlations between matrix metalloproteinase-2（ MMP-2） polymorphism（ 1306C/T and 735C/T） and the atherosclerotic cerebral infarction for the first time and recurrence. Methods The study consisted of 350 patients with atherosclerotic cerebral infarction for the first time onset,340 patients with a recurrence of atherosclerotic cerebral infarction,360 healthy subjects as control group. The serum level of MMP-2 was measured by enzyme-linked immunosorbent assay,and the MMP-2 1306 C / T and 735 C / T polymorphism were detected by PCR-restriction fragment length polymorphism. The difference results among the groups were analyzed. Results The serum MMP-2 level in cerebral infarction groups was significantly higher than that in the control group[（ 2. 93 ± 0. 53） vs（ 1. 68 ± 0. 24） mg / L,P 0. 01;（ 3. 32 ± 0. 94） vs（ 1. 68 ± 0. 24） mg / L,P 0. 01]. The serum MMP-2 level in the cerebral infarction recurrence group was significantly higher than those in the group of cerebral infarction for the first time onset[（ 3. 32 ± 0. 94） vs（ 2. 93± 0. 53） mg / L,P 0. 01]. The frequency of C allele of MMP-2 735 C / T in the cerebral infarction recurrence group was significantly higher than the control group（ 92. 5% vs 78. 5%,χ2= 8. 121; P 0. 01）,and there was no significantly difference in the cerebral infarction for the first time onset（ 92. 5% vs 87. 9%,χ2= 4. 398,P 0. 01）. There was also no significantly difference between the cerebral infarction for the first time onset and control group（ 87. 9% vs 78. 5%,χ2= 4. 121;P 0. 01）. There were no significantly difference in the frequencies of genotype（ χ2= 1. 075,P 0. 01） and allele（ χ2=1. 124,P 0. 01） of MMP-2 1306 C / T among the three groups. Multivariate Logistic regression analysis showed that MMP-2735 C / T polymorphism was an independent risk factor for infarction recurrence（ OR 1. 765,95% CI 1. 177 ~ 2. 676,P =0. 006）. Conclusions The C allele of MMP-2 735 C / T might be a genetic marker and an independent risk factor for atherosclerotic cerebral infarction recurrence.
出处
《中风与神经疾病杂志》
CAS
北大核心
2015年第2期104-107,共4页
Journal of Apoplexy and Nervous Diseases
基金
四川省遂宁市科知局项目(No.2014015)
