摘要
目的建立测定桂枝茯苓丸有效部位中苦杏仁苷、芍药苷质量分数的方法。方法采用Diamonsil C18（250 mm×4.6 mm,5μm）色谱柱;流动相为甲醇-水（体积比35∶65）;流速为1.0 m L·min^-1;检测波长为215 nm;柱温为30℃。结果苦杏仁苷和芍药苷的线性范围分别为0.354～7.088μg·m L^-1（r=0.999 5）和0.356～7.128μg·m L^-1（r=0.999 7）,平均回收率分别为98.48%（RSD=1.47%）和99.06%（RSD=1.49%）。结论本方法专属性强、操作简便,适用于桂枝茯苓丸中有效部位的质量控制。
Objective To establish the determination method for amygdalin and paeoniflorin in the active fraction of Guizhi Fuling pills by HPLC. Methods The Diamonsil C18（ 250 mm×4.6 mm,5 μm） was used with methanol-water（ 35 ∶65） as the mobile phase. The UV detection wavelength was set at 215 nm,the flow rate was 1.0 m L·min^-1,and the column temperature was 30 ℃. Results The linear range of amygdalin was0. 354-7.088 μg·mL^-1（ r = 0. 999 5）,and the average recovery was 98.48% with RSD of 1. 47%（ n = 6）.The linear range of paeoniflorin was 0. 356-7.128 μg·m L^-1（ r= 0. 999 7）,and the average recovery rate was99.06% with RSD of 1. 49%（ n = 6）. Conclusion The method was highly specific,simple,and could be used for the quality control of the active fraction in Guizhi Fuling pills.
出处
《广东药学院学报》
CAS
2015年第1期50-53,共4页
Academic Journal of Guangdong College of Pharmacy
基金
国家自然科学基金项目(81073024
81274060)
广东药学院师资培养计划项目
