摘要
目的：建立连翘药材中连翘新苷A、连翘酯苷A、松脂醇β-D葡萄糖苷、连翘苷和连翘脂素的含量测定方法。方法：采用高效液相色谱-二极管阵列检测法（HPLC-DAD）,采用Welch Ultimate C18（4.6mm×250mm,5μm）色谱柱,流动相为水（A）-乙腈（B）,梯度洗脱,流速1m L/min,柱温25℃。结果：连翘新苷A、连翘酯苷A、松脂醇β-D葡萄糖苷、连翘苷、连翘脂素进样浓度分别在7.81~500.00μg/m L,2.42~620.00μg/m L,1.21~620.00μg/m L,2.27~580.00μg/m L,0.59~600.00μg/m L之间有良好的线性关系。平均回收率分别为99.93%、99.95%、99.65%、99.52%、99.74%,RSD分别为2.09%、1.93%、1.17%、1.76%、0.88%。结论：该方法稳定性好,精密度高,重复性好,对于连翘药材的质量控制提供了理论依据。
Objective： To establish an HPLC method for simultaneous determination of Lianqiaoxinside A,forsythoside A,pinoresinol- β- D- glucoside,phillyrin and phillygenin of Forsythiae Fructus. Methods： The procedure of HPLC-PDA was performed on the chromatographic column of Welch Ultimate C18（ 4. 6mm × 250 mm,5μm） and the mobile phase of water（ A）- acetonitrile（ B） gradient elution. The flow rate was 1m L / min; the temperature was kept at 25℃.Results： Lianqiaoxinside A,forsythoside A,pinoresinol- β- D- glucoside,phillyrin and phillygenin were linear in the range of 7. 81 ~ 500. 00μg / m L,2. 42 ~ 620. 00μg / m L,1. 21 ~ 620. 00μg / m L,2. 27 ~ 580. 00μg / m L,0. 59 ~600. 00μg / m L respectively. The average recoveries were 99. 93%,99. 95%,99. 65%,99. 52% and 99. 74% respectively（ RSD 2. 09%,1. 93%,1. 17%,1. 76% and 0. 88%）. Conclusion： The method is accurate with higher repeatability,which can be used in the quality control of Forsythiae Fructus and its extract.
出处
《中医药学报》
CAS
2015年第1期47-49,共3页
Acta Chinese Medicine and Pharmacology
基金
国家科技重大专项重大新药创制(2012ZX09304005004)
黑龙江省博士后启动基金(LBH-Q13158)
黑龙江中医药大学校科研基金(No.035094)
