摘要
目的：建立牛黄解毒片多成分（黄芩苷、黄芩素、芦荟大黄素、大黄素）含量测定方法,完善牛黄解毒片质量控制标准。方法：以Agilent ZORBAX SB-C18（4.6mm×250mm,5μm）为色谱柱,检测波长：260nm,进样量：10μL,柱温：30℃,流速：1.0m L/min,以乙腈为流动相A,以0.1%磷酸为流动相B,进行梯度洗脱。结果：含量测定中黄芩苷、黄芩素、芦荟大黄素、大黄素的线性范围依次是5.06~202.4μg/m L、1.836~30.6μg/m L、0.196~7.84μg/m L、0.992~19.84μg/m L;相关系数R2依次是0.999 7、0.999 1、0.999 8、0.999 8;平均回收率分别是99.17%（RSD=0.72%）、97.46%（RSD=0.85%）、97.91%（RSD=1.97%）、98.23%（RSD=0.82%）（n=6）。结论：该方法准确度高,可以用于牛黄解毒片的质量评价。
Objective： To establish a method for the determination of multi- components（ baicalin,baicalein,aloe emodin and emodin） in Niuhuang Jiedu tablets（ NHJDT） and perfect the quality control standards. Methods： Using Agilent ZORBAX SB- C18（ 4. 6 × 250 mm,5μm） as the separation column,the UV absorbance was monitored at 260 nm by injecting 10μL of the sample solution each time and the column temperature was maintained at 30℃. The flow rate was1. 0m L / min. Acetonitrile was as the mobile phase A; 0. 1% phosphoric acid was as mobile phase B for gradient elution.Results： The linear range in content determination of baicalin,baicalein,aloe emodin and emodin was followed by5. 06 ~ 202. 4μg / m L,1. 836 ~ 30. 6μg / m L,0. 196 ~ 7. 84μg / m L and 0. 992 ~ 19. 84μg / m L,the correlation coefficient R2 was in turn 0. 999 7,0. 999 1,0. 999 8 and 0. 999 8. The average recoveries were 99. 17%（ RSD = 0. 72%）,97. 46%（ RSD = 0. 85%）,97. 91%（ RSD = 1. 97%）,98. 23%（ RSD = 0. 82%）（ n = 6）. Conclusion： The method is accurate and can be used to evaluate the quality of NHJDT.
出处
《中医药学报》
CAS
2015年第1期50-53,共4页
Acta Chinese Medicine and Pharmacology
基金
北京中医药大学研究生自主课题(2014-JYBZZ-XS-081)
中国药典2015版"中成药多组分控制方法的应用研究"
保健食品中违禁物质检测技术研究(2012BAK08B02)
关键词
HPLC
牛黄解毒片
含量测定
HPLC
Niuhuang Jiedu tablets
Content determination
