摘要
目的探讨RASSF2A基因启动子甲基化状态及其在子宫颈癌发生中的作用。方法采用甲基化特异性聚合酶链反应（MSP）和实时定量聚合酶链反应（RT-PCR）方法检测RASSF2A基因在62例子宫颈癌组织、30例正常子宫颈组织中的甲基化状态和表达情况。结果RASSF2A基因启动子区在子宫颈癌组织的甲基化比例高于正常子宫颈组织,分别为53.2 % （33／62）和13.3 %（4／30）,两组差异有统计学意义（P〈0.05）。子宫颈癌组织中RASSF2A基因mRNA表达低于正常子宫颈组织（mRNA相对表达量分别为0.254 3±0.102 7和0.623 4±0.063 8,t=0.932,P〈0.05）;在子宫颈癌组织中,甲基化组RASSF2A基因的mRNA表达低于非甲基化组（mRNA相对表达量分别为0.118 2±0.035 2和0.301 2±0.076 4）,差异有统计学意义（t=0.481,P〈0.05）。结论RASSF2A基因启动子甲基化是RASSF2A基因失活的主要机制,在子宫颈癌的发生过程中发挥重要作用。
ObjectiveTo investigate the methylation status of the promoter of RASSF2A gene in the tissues of cervical cancer and its impacts in tumorigenesis. MethodsMethylation-specific PCR （MSP） and RT-PCR techniques were applied to detect methylation status of RASSF2A gene promoter region and RASSF2A expression in tissues of 62 cervical cancer and 30 normal cervical specimens, respectively. ResultsMethylation frequency of RASSF2A gene in cancer tissue was higher than that in normal tissue （53.2 % vs 13.3 %, P 〈 0.05）. The mRNA expression of RASSF2A in cancer tissue were less than those in normal tissue significantly （0.254 3 ±0.102 7 vs 0.623 4 ± 0.063 8, t = 0.932, P 〈 0.05）. The mRNA expressed of RASSF2A gene was lower in methylated promoter group than that in unmethylated promoter group in cancer tissue samples significantly （0.118 2 ± 0.035 2 vs 0.301 2 ± 0.076 4, t = 0.481, P 〈 0.05）. ConclusionHypermethylation of RASSF2A gene in promoter region, which lead to the loss of RASSF2A gene expression, might play an important role in tumorigenesis of cervical cancer.
出处
《肿瘤研究与临床》
CAS
2015年第2期117-119,共3页
Cancer Research and Clinic
