摘要
为探究小麦雌蕊化现象的分子遗传机制,本文从CSTP和HTS-1 2个小麦品系的幼穗中克隆得到了2个B类MADS-box基因WPI1和WPI2。WPI1基因c DNA序列长816 bp,ORF长627 bp,编码208个氨基酸残基;WPI2基因c DNA序列长983 bp,ORF长630 bp,编码209个氨基酸残基。WPI1和WPI2基因均含有典型的MADS-MEF2-like和K-box结构域,C端均具有PI结构基序。系统进化树分析表明WPI1和WPI2基因属于PI/GLO亚家族成员。Real-time PCR分析表明WPI1和WPI2基因在CSTP和HTS-1小穗中的表达模式明显不同。雌雄蕊原基形成期,WPI1和WPI2基因在HTS-1中的表达量较高,而在CSTP中表达量较低,接近为0;药隔期,WPI1和WPI2基因在HTS-1中的表达水平明显低于CSTP中的表达。WPI1和WPI2基因表达模式的改变与HTS-1雌蕊化表型有关。本研究结果为进一步探讨B类基因WPI1和WPI2在HTS-1雌蕊化中的作用奠定了基础。
To study the molecular and genetic mechanisms were cloned from CSTP and HTS-1 in this study. Sequence of pistillody in wheat, two B-class genes, WPH and WPI2 alignment results showed that the cloned cDNA of WPI1 and WPI2 was 816 bp and 983 bp in length, respectively. WPH gene contains a 627 bp open reading frame （ORF） which encodes 208 amino acids. WPI2 gene contains a 630 bp open reading frame （ORF） which encodes 209 amino acids. Alignment of the predicted amino acid sequences from WPI1 and WPI2 genes, as well as with that from previously published subfamily members, revealed the MADS-MEF2-1ike and K-box domains. In the two WP! genes, the consensus PI motif was found in the C-terminal region. Phylogenetic tree reconstructed clearly showed that these proteins were classed into PI/GLO subgroup of B class MADS-box protein family. Real-time PCR analysis indicated that the expression patterns of WPH and WPI2 genes are significantly different in spikelet of CSTP and HTS-1. In pistil and stamen primordiuxn formation stage the expression levels of WPII and WPI2 are higher in HTS-1 than CSTP, the latter is close to zero. In contrast, in anther seperation stage the expression levels of WPH and WPI2 are lower in HTS-1 than CSTP, obviously. WPH and WPI2 genes may be bound up with pistillody of HTS-1. The study provides more insights for future research the functions of B-class genes WPH and WPI2.
出处
《核农学报》
CAS
CSCD
北大核心
2015年第2期221-228,共8页
Journal of Nuclear Agricultural Sciences
基金
国家自然科学基金(31301319)
教育部重点项目(211164)
