摘要
植物对阴离子和阴离子的吸引是植物括盐性调控的重要内容。为了阐明拟南芥对中性盐中阳离子和阴离子抗性机制,本试验以拟南芥nos1(nitric oxide synthase1)突变体为材料,用Na2SO4、Na Cl、K2SO4、KCl作为盐胁迫因子,研究了突变体的根弯曲百分率、根长、株高、鲜重、存活率以及渗透调节物质脯氨酸和丙二醛含量的变化。拟南芥nos1突变体在4种不同盐胁迫下,呈现出不同的抗性,Na2SO4和Na Cl中突变体的根长显著高于K2SO4和KCl处理的材料,KCl中突变体的株高和鲜重显著高于Na2SO4和Na Cl中。随着盐浓度升高,突变体存活率显著低于对照,KCl中突变体的存活率显著高于Na2SO4和Na Cl中。另外突变体在Na2SO4和Na Cl中脯氨酸含量和丙二醛含量显著高于K2SO4和KCl处理的样品。这些结果表明突变体对盐胁迫下的阳离子和阴离子抗性机制存在显著差异,阳离子相同时,突变体对SO42-盐胁迫更敏感,阴离子相同时,突变体对Na+盐胁迫更敏感。本试验为提高作物抗盐性和生物技术改良提供理论依据。
The responses of Arabidopsis nosl （nitric oxide synthasel） mutant to cation and anion from neutral salt were investigated, the resistance mechanisms in Arabidopsis nosl mutant were clarified. The root bending percentage, the length of root, the height of plant, the fresh weight, the survival rate, the content of proline and MDA （malondialdehyde） under different concentrations of Na2SO4. NaCI, K2SO4 and KC1 were analyzed. The resistance characteristics of Arabidopsis nosl mutant under four salts stresses were examined. The length of roots were significantly higher under Na2SO4 and NaCI stress than that of K2SO4 and KC1 stress. The survival rate were declined with the salt concentration increasing in nosl mutant compared with control. However, the plant height and the the fresh weight were higher under KCI stress than under Na2SO4 and NaC1 stress. The content of proline and MDA were significantly higher under Na2SO4 and NaC1 stress than of K2SO4 and KC1 stress. These results indicated that the resistance mechanisms were significance different between cation and anion in nosl mutant under different salts stresses. The nosl mutant is more sensitive to sulfate radical when the cation is same. However, the nosl mutant is more sensitive to sodium salt when the anion is same. The salinity in the soil is a major factor that constrained growth and yield in crops. Activity of iro-transport, toxicty and balance of iro were investigated under salinity stress by model plant and then shed light on adaptive strategies and survival methods coped with salinity stress, providing theoretical basis for elevating tolerance to salinity and tmproving biotechnology.
出处
《核农学报》
CAS
CSCD
北大核心
2015年第2期359-364,共6页
Journal of Nuclear Agricultural Sciences
基金
国家自然科学基金(31060063
31260094)
甘肃省自然科学基金项目(0803RJZA0510)
甘肃省高校基本科研业务费项目
关键词
一氧化氮(NO)
Na盐
K盐
离子通道
nitric oxide
sodium salt
potassium salt
arabidopsis nosl mutant
