摘要
遗传连锁图谱的构建是基因组研究中的重要环节,也是基因定位与克隆、分子标记辅助育种及数量性状定位等研究的基础,加之分子标记技术的蓬勃发展,为构建高密度遗传连锁图谱奠定了技术基础。对遗传图谱的构建步骤、分子标记的种类、作图群体的种类及近5年我国药用植物遗传图谱的研究进展进行了简要概述,并探讨了目前该领域研究中存在的主要问题及今后的研究方向。
Genetic linkage mapping is one of the key steps in genomic research, which is the cornerstone of a wide spectrum of biotechnology applications, including gene localization, map-assisted cloning, molecular marker-assisted selection in breeding, and quantitative trait loci. Furthermore, the prospering of the molecular marker technology laid the technological foundation of constructing high density genetic linkage map. This paper briefly reviewed the procedure of genetic mapping, types of molecular markers, mapping populations, and research progress in construction of the genetic linkage maps of medicinal plants in recent five years. In addition, the existing problems and the future direction have been discussed in this field.
出处
《中草药》
CAS
CSCD
北大核心
2015年第3期435-442,共8页
Chinese Traditional and Herbal Drugs
基金
国家自然科学基金项目(31070298
31470407)
浙江省科技计划项目(2014C32090)
浙江省自然科学基金项目(LQ13H280006)
浙江省药用植物种质改良与质量控制技术重点实验室开放项目(201304)
浙江省大学生科技创新活动计划暨新苗人才计划(ZX13005002067)
关键词
药用植物
遗传连锁图谱
分子标记
辅助育种
目的基因定位和克隆
medicinal plant
genetic linkage map
molecular marker
assistant breeding
gene localization and cloning