摘要
目的:分析CD31、CD105和PTEN蛋白与宫颈癌临床病理指标的关系,探讨其在宫颈癌发生、发展、转移及预后中的意义及相互关系。方法:采用免疫组化SP法检测65例宫颈癌组织、25例正常宫颈组织中的CD31、CD105标记微血管密度(MVD)情况和PTEN蛋白表达情况。结果:CD31在宫颈癌组织中MVD为(48.33±3.66)高于正常宫颈组织中MVD(8.68±1.26),差异有统计学意义(P<0.05);CD31-MVD值与宫颈癌组织学类型、临床分期有关(P<0.05),而与年龄、淋巴结转移及组织学分化程度无关(P>0.05)。CD105在宫颈癌组织中MVD为(32.56±2.81)高于正常宫颈组织中MVD(1.69±2.14),差异有统计学意义(P<0.05);MVD值与宫颈癌临床分期、有无淋巴结转移有关(P<0.05),而与年龄、组织学分化程度及组织学类型无关(P>0.05)。正常宫颈组织PTEN阳性表达率为100.0%(25/25)高于宫颈癌组织36.92%(24/65),差异有统计学意义(P<0.01)。宫颈癌组织PTEN阳性表达与临床分期、淋巴结转移有关(P<0.05),而与年龄、分化程度、组织类型等无关(P>0.05)。宫颈癌组织中CD31和CD105的表达呈正相关(rs=0.83,P<0.05),CD31和PTEN呈负相关(rs=-0.68,P<0.05),CD105与PTEN呈负相关(rs=-0.76,P<0.05)。结论:联合检测PTEN、CD31与CD105可作为宫颈癌诊断、治疗、转移及评估预后的重要指标。
Objective： To analyze the relationship between CD31,CD105,PTEN and clinical pathological parameters of cervical cancer,explore their significance and interaction in occurrence,development,metastasis,and prognosis of cervical cancer. Methods： Immunohistochemical SP method was used to detect CD31 / CD105 marked microvessel density（ MVD） and PTEN expression in 65 patients with cervical cancer and 25 patients with normal cervical tissue. Results： MVD of CD31 in cervical cancer was（ 48. 33 ± 3. 66）,which was statistically significantly higher than that in normal cervical tissue（ 8. 68 ± 1. 26）（ P〈0. 05）. CD31- MVD was correlated with histological types of cervical cancer and TNM stages（ P〈0. 05）,which was not correlated with age,lymph node metastasis,and histological differentiation degree（ P〉0. 05）. MVD of CD105 in cervical cancer was（ 32. 56 ± 2. 81）,which was statistically significantly higher than that in normal cervical tissue（ 1. 69 ± 2. 14）（ P〈0. 05）. CD105- MVD was correlated with clinical staging and lymph node metastasis（ P〈0. 05）,which was not correlated with age,histological differentiation degrees and histological types（ P〉0. 05）. The positive expression rate of PTEN in normal cervical tissue was 100. 0%（ 25 /25）,which was statistically significantly higher than that in cervical cancer（ 36. 92%,24 /65）（ P〈0. 01）. PTEN positive expression in cervical cancer was correlated with clinical staging and lymph node metastasis（ P〈0. 05）,which was not correlated with age,histological differentiation degrees,and histological types（ P〈0. 05）. In cervical cancer,CD31 expression was positively correlated with CD105 expression（ rs= 0. 83,P〈0. 05）,CD31 expression was negatively correlated with PTEN expression（ rs=- 0. 68,P〈0. 05）,CD105 expression was negatively correlated with PTEN expression（ rs=- 0. 76,P〈0. 05）. Conclusion： Joint detection of PTEN,CD31,and CD105 can be used as important parameters for diagnosis,treatment,metastasis,and prognosis assessment of cervical cancer.
出处
《中国妇幼保健》
CAS
2015年第9期1446-1449,共4页
Maternal and Child Health Care of China
